| 比色法测定不同产地傣药肾茶中主要酚类物质含量 |
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| 引用本文： | 辛雪,;董霄,;张彪,;曹酌中,;邹海淼,;苏靖,;邱明丰.比色法测定不同产地傣药肾茶中主要酚类物质含量[J].国际药学研究杂志,2014(3):368-373. |
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| 作者姓名： | 辛雪 ;董霄 ;张彪 ;曹酌中 ;邹海淼 ;苏靖 ;邱明丰 |
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| 作者单位： | [1]上海交通大学药学院,上海200240; [2]上海交通大学医学院,上海200025 |
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| 基金项目： | 上海市科委项目（13401900800）;云南省科技厅项目（2008IF018） |
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| 摘 要： | 目的：优选合适的显色体系，建立傣药肾茶中主要酚类物质的含量测定方法，对不同产地样品进行含量测定。方法以迷迭香酸为对照品、亚硝酸钠-硝酸铝-氢氧化钠为显色剂、检测波长为500 nm、采用比色法测定19个不同产地样品的主要酚类物质含量。结果迷迭香酸在8.792~17.584μg/ml范围内线性关系良好（r2=0.9993），平均加样回收率为100.73%，相对标准偏差（RSD）为0.85%。不同产地样品中主要酚类物质的质量分数为1.16%~3.49%。结论该方法简单、准确、重复性好，适合傣药肾茶中主要酚类物质的含量测定。结果显示不同产地样品中以云南产肾茶中主要酚类物质含量相对较高。
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| 关 键 词： | 肾茶 主要酚类物质 含量测定 比色法 |
Determination of the main phenolic in Clerodendranthus spicatus from different areas by colorimetric method |
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| Institution: | XIN Xue, DONG Xiao, ZHANG Biao, CAO Zhuo-zhong, ZOU Hai-miao, SU Jing, QIU Ming-feng ( 1. School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China ; 2. School of Medicine, Shanghai Jiaotong University, Shanghai 200025,China) |
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| Abstract: | Objective To choose an appropriate coloured system and develop a method for the determination of the main phenolic in Dai Medicine -Clerodendranthus spicatus from different areas. Methods The main phenolic in Clerodendranthus spicatus from 19 areas was determined at 500 nm wavelength, with rosmarinic acid as reference and sodium nitrite-aluminum nitrate-sodium hydroxide as colour-developing system. Results Rosmarinic acid presented good linear relationship （r2=0.9993） in the concentration range of 8.792-17.584μg/ml.The average recovery was 100.73%and RSD was 0.85%.The content of the main phenolic in Clerodendranthus spicatus ranged from 1.16 % to 3.49 %. Conclusion The method is appropriate for the determination of the main phenolic in Clerodendranthus spicatus. The main phenolic content in samples from Yunnan province is relatively high. |
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| Keywords: | Clerodendranthus spicatus main phenols determination colorimetric method |
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