HPLC梯度洗脱法同时测定参杞酒中的党参炔苷、东莨菪苷、东莨菪亭、槲皮素、山柰酚和异鼠李素

目的：建立HPLC梯度洗脱法同时测定参杞酒中的党参炔苷、东莨菪苷、东莨菪亭、槲皮素、山柰酚和异鼠李素。方法采用Shiseido C18（4.6 mm×250 mm，5μm）色谱柱；流动相A：乙腈，流动相B：0.5％醋酸水溶液，进行梯度洗脱；进样量为20μl；流速：1.0 ml/min；柱温：30℃；检测波长分别为269 nm（党参炔苷）、346 nm（东莨菪苷和东莨菪亭）和360 nm（槲皮素、山柰酚和异鼠李素）。结果党参炔苷、东莨菪苷、东莨菪素、槲皮素、山柰酚和异鼠李素分别在4.59～91.80（r=0.9999）、2.62～52.40（r=0.9996）、1.95～39.00（r=0.9998）、3.34～66.80（r=0.9995）、2.30～46.00（r=0.9991）、2.86～57.20μg/ml（r=0.9999）范围内与峰面积呈良好的线性关系，平均加样回收率分别为98.27％、97.62％、99.17％、98.50％、97.35％和98.86％，RSD（n=9）分别为1.33％、1.48%、0.99％、1.37%、1.35％、1.70%。结论本文建立的HPLC梯度洗脱法同时测定参杞酒中的上述6种组分，方法操作简便，准确，重现性好，可作为参杞酒全面可靠的质量控制方法。

Determination of lobetyolin,scopolin,scopoletin,quercetin,kaempferol and isorhamnetin in Shenqi Jiu by HPLC with gradient elution method

Objective To develop an HPLC method with gradient elution for determination of lobetyolin,scopolin,scopole?tin,quercetin,kaempferol and isorhamnetin in Shenqi Jiu. Methods A Shiseido C18 column(4.6 mm×250 mm,5μm)was adopted, the mobile phase was acetonitrile(A)-0.5%acetic acid solution(B)with gradient elution at a flow rate of 1.0 ml/min,the column tem?perature was set at 30℃,the sample quantity was 20μl,and the detection wavelengths were 269(lobetyolin),346(scopolin and sco?poletin)and 360 nm(quercetin,kaempferol and isorhamnetin). Results The linear ranges of above mentioned 6 ingredients in order fell within the range of 4.59-91.80(r=0.9999),2.62-52.40(r=0.9996),1.95-39.00(r=0.9998),3.34-66.80(r=0.9995),2.30-46.00 (r=0.9991),and 2.86-57.20μg/ml(r=0.9999),respectively. The average recoveries and RSD(n=9)were 98.27%(1.33%),97.62%(1.48%),99.17%(0.99%),98.50%(1.37%),97.35%(1.35%),and 98.86%(1.70%),respectively. Conclusion The established HPLC gradient elution method can simultaneously determine the above mentioned 6 ingredients Shenqi Jiu. The method is simple,ac?curate,with good reproducibility. The method is helpful for the quality control of Shenqi Jiu.