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| 采用模式生物斑马鱼评价三种中药成分的急性毒性 | |
| 引用本文： | 舒斌,韦英杰,张陆勇,江振洲.采用模式生物斑马鱼评价三种中药成分的急性毒性[J].云南中医学院学报,2010,33(1):35-37. |
| 作者姓名： | 舒斌 韦英杰 张陆勇 江振洲 |
| 作者单位： | 1. 中国药科大学新药筛选中心,江苏南京,210009;南京工业大学江苏省药物研究所,江苏南京,210009 2. 江苏省中医药研究院,江苏南京,210028 3. 中国药科大学新药筛选中心,江苏南京,210009 4. 中国药科大学江苏省药效研究与评价服务中心,江苏南京,210009 |
| 基金项目： | 财政部2007年度公益性行业科研专项 |
| 摘 要： | 目的：以模式生物斑马鱼为实验对象，观察3种中药有效成份雷公藤甲素、大黄素和苦参碱的急性毒性。方法：将药物添加到鱼只生活的水中为给药方式，观察给药后24h鱼只的死亡情况，以此为判断待测药物毒性大小的依据，并用Bliss法计算各药物对斑马鱼的半数致死浓度（LC50）。结果：雷公藤甲素、苦参碱和大黄素对于斑马鱼的LC50值依次为5．02×10^-3、113．2和1．18×10^3μg／mL，毒性大小顺序与文献报道基本一致。结论：以斑马鱼作为初步评价药物毒性的模型可用于中药成分毒性的快速筛选和评价。 |
| 关 键 词： | 斑马鱼 雷公藤甲素 苦参碱 大黄素 LC50 |
Acute Toxicity of Triptolide,Matrine and Emodin on Zebrafish |
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| SHU Bin,WEI Ying-jie,ZHANG Lu-yong,JIANG Zhen-zhou.Acute Toxicity of Triptolide,Matrine and Emodin on Zebrafish[J].Journal of Yunnan College of Traditional Chinese Medicine,2010,33(1):35-37. | |
| Authors: | SHU Bin WEI Ying-jie ZHANG Lu-yong JIANG Zhen-zhou |
| Institution: | SHU Bin, WEI Ying-jie, ZHANG Lu -yong, JIANG Zhen -zhou ( 1. Center for Drug screening, China Pharmaceutical University, Nanjing Jiangsu 210009 ; 2. Jiangsu Provincial Institute of Meteria Medica, Nanjing University of Technology, Nanjing Jiangsu 210009 ; 3. Jiangsu Provincial Institute of Traditional Chinese Medicine, Nanjing Jiangsu 210028 ; 4. Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University, Nanjing Jiangsu 210009) |
| Abstract: | To observe the acute toxicity of triptolide, matrine and emodin on zebra：fish. Methods： the three compounds were added into the water where zebrafish lives, and the 50% lethal concentrations （LC50） were measured according to the death rate of the fish. Results ： The LC50 of triptolide, matrine and emodin on zebrafish were 5. 02 × 10^-3, 113.2 and 1.18 × 10^3μg/ml, respectively; the toxicity order was similar to that reported. Conclusion： The safety of compounds was able to be evaluated on the zebrafish model. |
| Keywords: | LC50 |
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