消异汤联合达那唑对大鼠异位子宫内膜组织VEGF及MVD表达的影响

目的：探讨消异汤联合达那唑对大鼠异位子宫内膜组织血管内皮生长因子VEGF及微血管密度MVD表达的影响。方法：采用自体移植法改良建立Wistar大鼠子宫内膜异位症模型,4周后将建模成功的大鼠随机分为生理盐水对照组（U）,消异汤组（XYT）,达那唑组（D）,和联合用药小剂量组（SS）,联合用药常规剂量组（CS）,每组10只,灌药4周后,择动情期处死大鼠。采用免疫组化法测定血管内皮生长因子（VEGF）的表达,并通过CD31标记异位子宫内膜血管,测定其微血管密度（MVD）。结果：消异汤组、达那唑组及各联合用药组VEGF和MVD的表达均低于对照组（P〈0.05）,联合用药常规剂量组最低,其中达那唑组和联合用药小剂量组间比较差异无统计学意义。结论：消异汤可降低子宫内膜异位症模型大鼠的VEGF及MVD的表达水平,消异汤与达那唑合用,不仅可以提高疗效,而且可以适当降低达那唑的用药剂量。

Effect of Xiaoyitang and Danazol on the Expression of VEGF and MVD in Ectopic Endometrial Tissue in Rats

Objective： To explore the effect of Xiaoyitang and danazol on the expression of VEGF and MVD in ectopic endometrial tissue in female rats with endometriosis.Methods： Wistar rats endometriosis models were established,successful endometriosis rats were divided into control group,XiaoYiTang group,danazol group,minimal combination group,medium combination group,large combination group.Ectopic endometrium grafts volume and histomorphology were observed,vescular endothelial growth factor（VEGF） was observed in ectopic endometrium by immunohistochemistry（SABC technique）.MVD was determined by immunostaining for CD31.Results： It was confirmed with pathology that the rat model was made successfully by autotransplantation of its own endometrium between the superficial and muscular layer of the abdomial wall.Ectopic endometrium grafts volume has no significant difference in six group before treatment（P 0.05）.there was diminished significantly after treatment（P0.05）,which effects are dose-dependent.MVD and the level of VEGF in the ectopic endometrium was decreased significantly after treatment,which effects are dose-dependent.And ectopic endometrium grafts volume,or MVD,or the level of VEGF has no significant difference between danazol group and minimal combination group after treatment.Conclusion： XiaoYiTang can decrease the quantity the microvessel and the expression of protein VEGF in heterotopic endometrium of endometriosis rats.XiaoYiTang not only can improve the curative effect of danazol but also can reduce the dose of danazol when combining with danazol.