电针对缺血性脑卒中大鼠记忆功能及脑内突触囊泡蛋白的影响

观察电针对缺血性脑卒中大鼠记忆功能及脑内突触囊泡蛋白（synaptic vesicular protein，SYN）的影响，探讨其可能的作用机制。方法 选取90 只SD大鼠随机分为模型组、电针组、假手术组，每组各30只。线拴法制作急性大脑中动脉缺血大鼠模型。电针组取“百会”“水沟”“内关”“三阴交”穴位，应用“醒脑开窍”法进行电针，每天电针30 min，连续电针6 d休1 d，7 d为一个疗程，首次电针干预在造模成功24 h后进行。模型组和假手术组不进行电针干预。大鼠分别按7、14、21 d 3个亚组进行运动及记忆功能评分，TTC染色测定脑梗死体积、Western blot检测脑内SYN的蛋白表达。结果 电针组运动功能评分较模型组显著降低（P<0.01），电针组进入隐藏区潜伏期时间较模型组显著缩短（P<0.01），电针组脑梗死率较模型组显著缩小（P<0.01），SYN的蛋白表达电针组较模型组明显增强（P<0.01）。假手术组无神经功能缺损及脑梗死灶，SYN表达最弱。结论 电针干预能减小脑梗死体积，上调脑内SYN的表达，进而促进缺血性脑卒中大鼠的记忆及运动等神经功能的恢复。

Effect of Electroacupuncture on Memory Function and Synaptic Vesicular Protein in Ischemic Stroke Rats

Objective To observe the effect of electroacupuncture on memory function and synaptic vesicular protein (SYN) in the brain of rats with ischemic stroke then explore its possible mechanism. Methods 90 SD rats were randomly divided into model group, electroacupuncture group and sham operation group. The rat model of acute middle cerebral artery ischemia was made by thread tying. In the electroacupuncture group, Baihui, Shuigou, Neiguan and Sanyinjiao acupoints were treated with the method of Xingnao Kaiqiao. The electroacupuncture lasted for 30 min every day, one day off for 6 consecutive days and one course continued for 7 d. The first electroacupuncture intervention was treated after 24 h of successful modeling. Model group and sham operation group did not receive electroacupuncture intervention.10 rats in the 3 groups were randomly divided into 3 subgroups: 7, 14, 21 d groups. The motor and memory function scores were measured. The cerebral infarction rate was measured by TTC staining and the expression of SYN in the brain was detected by Western Blot. Results The score of motor function in electroacupuncture group was significantly lower than that in model group(P<0.01), the latency time of entering the hidden area in electroacupuncture group was significantly shorter than that in model group(P<0.01), the cerebral infarction rate in electroacupuncture group was significantly smaller than that in model group (P<0.01), and the expression of SYN in electroacupuncture group was significantly higher than that in model group(P<0.01). In sham operation group, there was no neurologic defect or cerebral infarction, and SYN expression was the weakest. Conclusion Electroacupuncture could reduce the volume of cerebral infarction, up regulate the expression of SYN in the brain, and then promote the recovery of neurological functions such as memory and motor in rats with ischemic stroke.