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注射用地龙冻干粉针对大鼠颈动脉血栓形成的影响
引用本文:梁超,刘冬洁,高瑞辰,韩丽敏,曲轶,沈鑫,朱久新,侯云龙,乔国芬.注射用地龙冻干粉针对大鼠颈动脉血栓形成的影响[J].张家口医学院学报,2011(5):14-18.
作者姓名:梁超  刘冬洁  高瑞辰  韩丽敏  曲轶  沈鑫  朱久新  侯云龙  乔国芬
作者单位:[哈尔滨医科大学附属第一医院,黑龙江,15008,中国 [1] 大庆油田总医院肿瘤科,黑龙江,163000,中国 [2] 哈尔滨医科大学药理教研室,黑龙江,150081,中国 [3],黑龙江,150081,中国
基金项目:黑龙江省教育厅科学技术研究项目(No.L12511320),哈尔滨医科大学大学生创新创业训练计划
摘    要:目的:评价注射用地龙冻干粉针的抗血栓作用。方法:SD雄性大鼠40只,利用血栓生成仪复制颈动脉血栓模型,模型成功后72h,将动物随机分成空白组、地龙冻干粉针1.8,0.9mg·kg^-1剂量组及阳性药疏血通注射液对照组,每组lO只,各组大鼠分别尾静脉注射(iv)生理盐水、地龙冻干粉针1.8,0.9mg·kg^-1剂量溶液和疏血通注射液(O.1mL·kg^-1)12d后,剥离颈动脉血栓,测定血栓重量,并对各组大鼠腹主动脉取血,利用ELISA法测定血浆组织型纤溶酶原激活剂(tissue type plasminogen activator,t-PA)含量,采用观察法测定各组大鼠的凝血时间(clotting time,CT)、血浆复钙时间(recalcification time,RT),利用血小板聚集仪测定血小板最大聚集率(maximal platelet aggregation rate,PAgR-max)。结果:与空白组相比较,地龙冻干粉针1.8mg·kg^-1剂量组大鼠血栓重量显著减少(P〈0.05);阳性药疏血通组与地龙冻干粉针0.9mg·kg^-1剂量组大鼠血浆t-PA含量显著升高(P〈0.05);阳性药疏血通组、地龙冻干粉针1.8mg·kg^-1剂量组大鼠CT、RT显著延长(P〈0.05),0.9mg·kg^-1剂量组RT显著延长(P〈0.05),且地龙冻干粉针对RT延长呈剂量依赖性。结论:注射用地龙冻干粉针具有显著的溶解血栓的作用,并能够抑制内源性凝血途径,溶血栓作用可能与提高血浆t-PA含量有关。

关 键 词:地龙冻干粉针  颈动脉血栓形成  抗血栓

Anti-thrombotic Effect of Lyophilized Earthworm Powder for Injection
Authors:LIANG Chao  LIU Dong-jie  GAO Rui-chen  HAN Li-min  QU Yi  SHEN Xin  ZHU Jiu-xin  HOU Yun-long  QIAO Guo-fen
Institution:1The First Affiliated Hospital of Harbin Medical University, Harbin, 150081, China 2Department of Ontology, the General Hospital of Daqing Oil Field, Daqing, 163000, China ;3Department of Pharmacology, Harbin Medical University, Harbin, 150081, China)
Abstract:Objective:To observe the anti-thrombotic effects of lyophilized earthworm powder for injection (LEPI). Methods:Rats were randomly divided into four groups:blank control,low dose ELPI, high dose ELPI and Shuxuetong injection group. Carotid artery thrombus was induced by a thrombus formation instrument. Seventy-two hours after thrombus induction, rats were treated with saline (2 mL·kg^-1 ),low dose of LEPI (0.9 mg·kg^-1 ),high dose of LEPI (1.8mg·kg^-1), and Shuxuetong solution (0.1 mL·kg^-1 ) , respectively, daily for 12 consecutive days. Blood was sampled for plasma t-PA level determination using ELISA method,CT and RT values were measured in all groups,and the maximal platelet aggregation rate (PAgt~-max) was tested using LBY-NJ4 method. Results:Compared with control group,thrombus weight in high dose LEPI group was decreased significantly (P〈0.05 ); plasma t-PA levels in both low dose and positive control groups were significantly elevated (P〈0.05); CT and RT in Shuxuetong treated control group and high-dose LEPI group were significantly prolonged (P〈0.05);RT was dose- dependently prolonged by LEPI treatment. Conelusion:LEPI possesses significant thrombolytic effects, possibly by inhibiting intrinsic coagulation pathway through increasing plasma level of t-PA.
Keywords:lyophilized earthworm powder for injection  carotid artery thrombosis  anti-thrombosis
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