兔骨髓基质干细胞体外培养的实验研究

目的：探讨兔骨髓基质干细胞的体外培养技巧，观察免骨髓基质干细胞的体外生长特性，为用于骨组织工程的功能细胞提供实验基础。方法：自兔髂骨抽取骨髓，分离骨髓基质干细胞，分别采用常规培养液及含矿化液的培养液进行培养，绘制细胞生长曲线，对细胞行碱性磷酸酶染色，计算细胞碱性磷酸酶染色阳性率，观察细胞在体外长期培养时钙结节形成情况。结果：第1-3代细胞生长曲线基本相同，加入矿化液后细胞生长速度明显减慢，但细胞表现出成骨细胞特性，其碱性磷酸酶染色阳性率大大提高，在体外长期不传代培养时可形成钙结节。结论：兔骨髓基质干细胞在体外适当的培养条件下可以向成骨细胞方向分化，可作为骨组织工程的种子细胞．

Investigation on the cultivation of rabbit marrow stem cell in vitro

Objective To investigate the method of culturing rabbit marrow stem cell(MSC)and observe its growing character.Methods Bone marrow was extracted from the iliac of rabbit.MSC was then separated from the marrow and cultured with DMEM media and DMEM mineralized liquid respectively.The growth curve,ALP stain and calcification nodule formed by MSC were observed.Results Growth curves of passage 1-3 MSC had the similar characters and when the MSC was cultured with mineralized media.It showed the character of osteoblast,such as positive ratio of ALP stain,increased greatly and could form calcification nodule.Conclusion Rabbit MSCs differentiate into osteoblasts when cultured in vitro,therefore they can be used as the seed cells for the bone tissue engineering.