羧甲基赖氨酸对人外周血单核细胞破骨样转化的影响

目的：探讨羧甲基赖氨酸（ CML）对外周血单核细胞破骨样转化的影响。方法：从外周血分离提取单核细胞，分为3组：细胞核因子κB受体活化因子配基（ RANK-L）＋巨噬细胞集落刺激因子（ M-CSF）诱导组（ A组），CML＋RANK-L＋M-CSF干预组（ B组），空白对照组（ C组）。利用RANK-L和M-CSF诱导其破骨样转化，每天通过倒置显微镜观察细胞生长形态及计算细胞核个数，培养8d后通过抗酒石酸酸性磷酸酶（TRAP）染色法观察破骨样细胞的形成，Western blotting 检测破骨细胞标志物组织蛋白酶K蛋白表达。结果：随着培养时间的延长，A组TRAP阳性多核细胞数明显增加，B组TRAP阳性多核细胞数较A组明显减少（P＜0．05），B组组织蛋白酶K表达亦较A组明显降低（P＜0．05）。结论：CML能够抑制外周血单核细胞向破骨样细胞转化。

Effects of N ^ε- （Carboxymethyl）lysine on human peripheral blood mononcytes differentiation to osteoclast-like cells

Objective：To investigate the effects of Nε-（Carboxymethyl）lysine（CML）on osteoclast-like differenti-ation in human peripheral blood monocytes （PBMCs）.Methods：PBMCs were isolated from blood of healthy donors by centrifugation on Ficoll-Hypaque Plus .Cells were inducted differentiation into osteoclasts in the presence of receptor activator of nuclear factor kappa-B ligand（RANKL） and macrophage colony stimulating factor （M-CSF）. After 8 d culture, morphologic characteristics and multinucleated osteoclast-like cell formation were observed through inverted microscope .Tartrate-resistant acid phosphatase （ TRAP ） positive cells were stained by using a TRAP staining kit . Cathepsin K protein expression was examined by Western blotting . Results： With the prolongation of the culture time , a great amount of multinucleated and TRAP positive cells could be found in induction group . Multinucleated and TRAP positive cells decreased significantly in CML treatment group . Conclusion：CML suppressed osteoclast-like differentiation in human PBMCs .