巨大儿胎盘组织中HO-1、IGF-1的变化及其意义

目的：探讨血红素氧合酶-1（HO-1）、胰岛素样生长因子-1（IGF-1）在健康母亲和妊娠糖尿病（GDM）母亲分娩的巨大儿胎盘组织中的变化及其意义。方法：选择足月新生儿60例，其中GDM母亲分娩的巨大儿（巨大儿A组）12例，健康母亲分娩的巨大儿组（巨大儿B组）18例，正常出生体重新生儿（对照组）30例。分别用免疫组织化学法观察胎盘HO-1、IGF-1的表达，用酶联免疫吸附法测定胎盘HO-1、IGF-1水平。结果：巨大儿A组、B组胎盘HO-1、IGF-1表达强度均高于对照组（ P＜0．05），巨大儿A组、B组与对照组比较，胎盘HO-1、IGF-1水平均增高（ P＜0．05），而巨大儿A组与B组之间HO-1、IGF-1的表达强度及水平比较，差异无统计学意义（P＞0．05）。巨大儿A组及B组HO-1与IGF-1的水平均呈正相关（P＜0．05）。结论：胎盘HO-1、IGF-1的表达水平可能与巨大儿的发生有关。

Changes and significance of placental heme oxygenase-1, insulin-like growth factor-1 levels in placenta tissue of macrosomias

Objective：To investigate the changes of heme oxygenase-1（HO-1）,insulin-like growth factor-1（IGF-1） in healthy macrosomia placenta and macrosomia of gestational diabetic placenta and it＇s significance .Methods： Sixty healthy full-term neonates were chosen and 12 cases of macrosomias who＇s mothers were gestational diabetes mellitus （ GDM ） patients as macrosomia group A , 18 cases of macrosomias who＇s mothers were healthy as macrosomia group B ,30 cases of normal birth weight neonates were as control group .The expression of HO-1 ,IGF-1 in placenta tissue were detected by the method of immunohistochemistry ,the levels of HO-1,IGF-1 in placenta tissue were measured by enzyme linked immunosorbent assay .Results：The intensity of HO-1,IGF-1 expression in placenta of both macrosomia group A and macrosomia group B were higher than at in control group （all P〈0.05）, the comparison of HO-1 ,IGF-1 levels in plaecnta of both macrosomia group A and macrosomia group B were higher than at in control group（all P〈0.05）.There were no significant difference was found between macrosomia group A and macrosomia group B（P〉0.05）.Both macrosomia group A and macrosomia group B,the significant positve correlations were observed between levels of HO-1 and levels of IGF-1 （ P〈0 .05 ） .Conclusion： The expression levels of placental HO-1,IGF-1 may have relationship with macrosomias .