| ADAM9基因真核表达质粒的构建及表达 |
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| 引用本文： | 乔纯忠,卞倩,戚宇华,焦永军,李俊生.ADAM9基因真核表达质粒的构建及表达[J].东南大学学报(医学版),2011,30(3):482-485. |
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| 作者姓名： | 乔纯忠 卞倩 戚宇华 焦永军 李俊生 |
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| 作者单位： | 1. 东南大学,医学院,江苏,南京,210009
2. 江苏省疾病预防控制中心,病原微生物研究所,江苏,南京,210009 |
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| 基金项目： | 江苏省自然科学基金资助项目 |
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| 摘 要： | 目的:构建人源性去整合素金属蛋白酶9(ADAM9)基因真核表达载体并表达其编码蛋白(转染293T细胞).方法:利用包含ADAM9全长编码核酸序列的质粒PCR4-TOPO作为模板,用PCR扩增其核心编码区,将其克隆至PMD18-T Vector中构建PMD18-T-ADAM9质粒,同时双酶切PMD18-T-ADAM9及P...
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| 关 键 词： | 去整合素金属蛋白酶9 真核表达载体 293T 免疫印记 |
Construction and eukaryotic expression of ADAM9 gene |
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| QIAO Chun-zhong,BIAN Qian,QI Yu-hua,JIAO Yong-jun,LI Jun-sheng.Construction and eukaryotic expression of ADAM9 gene[J].Journal of Southeast Univ: Medical Sci Ed,2011,30(3):482-485. |
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| Authors: | QIAO Chun-zhong BIAN Qian QI Yu-hua JIAO Yong-jun LI Jun-sheng |
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| Institution: | QIAO Chun-zhong1,BIAN Qian2,QI Yu-hua2,JIAO Yong-jun2,LI Jun-sheng1(1.Medical School,Southeast University,Nanjing 210009,China,2.Institute of Pathogenic Microbiology,Jiangsu Provincial Center for Disease Prevention and Control,China) |
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| Abstract: | Objective: To construct human a disintegrin and metalloprotease 9(ADAM9) gene into a eukaryotic expression vector PXJ40-HA and study the expression of ADAM9 gene in transfected 293T cells.Methods: ADAM9 gene was amplified from a vector PCR4-TOPO ordered from Open Biosystems and cloned into PMD18-T vector to construct a vector named PMD18-T-ADAM9.The PMD18-T-ADAM9 and the PXJ40-HA double digested by the same restrict enzymes,the recombinant eukaryotic expression vector PXJ40-HA-ADAM9 subsequently yielded.The... |
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| Keywords: | 293T |
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