| 聚合酶链反应检测淋球菌的临床应用 |
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| 引用本文： | 李明发,项凤青,郑谷,蒋清,戴玉田,熊晓明,单春梅.聚合酶链反应检测淋球菌的临床应用[J].东南大学学报(医学版),1995(4). |
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| 作者姓名： | 李明发 项凤青 郑谷 蒋清 戴玉田 熊晓明 单春梅 |
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| 作者单位： | 南京铁道医学院生物学教研室，南京市鼓楼医院泌尿外科，盐城市第一人民医院 |
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| 摘 要： | 应用ＰＣＲ法对１１３例临床标本中淋球菌菌体内所特有的４．２ｋｂ隐蔽性质粒上的ｃｐｐＢ基因进行了检测。与直接涂片法相比，ＰＣＲ法的敏感性和特异性分别达１００％和８７．５％。
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| 关 键 词： | 聚合酶链反应，淋病双球菌 检测 |
CLINICAL APPLICATION OF DETECTION OF NEISSERIA GONORRHOEAE BY POLYMERASE
CHAIN REACTION |
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| Li Mingfa, Xiang Fengqing, Zhen Gu, Jiang qing.CLINICAL APPLICATION OF DETECTION OF NEISSERIA GONORRHOEAE BY POLYMERASE
CHAIN REACTION[J].Journal of Southeast Univ: Medical Sci Ed,1995(4). |
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| Authors: | Li Mingfa Xiang Fengqing Zhen Gu Jiang qing |
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| Abstract: | PCR assay was performed on 113
clinical samples to detect the cppB gene of the plasmidwhich is specific for all Neisseria
gonorrhoeae and approximately 4.2 kb in size;Comparing withthe direct smear assay,the PCR
has a sensitibility of 100%and specificity of 87.5%. |
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| Keywords: | polymerase chain reaction neisseria gonorrhoeae detection |
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