| 纳米雄黄脂质体的制备、特性检测和体外抗肿瘤细胞作用的研究 |
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| 引用本文： | 王子妤,王丽,张东生.纳米雄黄脂质体的制备、特性检测和体外抗肿瘤细胞作用的研究[J].东南大学学报(医学版),2009,28(3):175-179. |
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| 作者姓名： | 王子妤 王丽 张东生 |
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| 作者单位： | 1. 南京中医药大学,病理学教研室,江苏,南京,210046;东南大学,临床医学院,江苏,南京,210009
2. 东南大学基础医学院,病理学与病理生理学系,江苏,南京,210009 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，南京中医药大学青年基金 |
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| 摘 要： | 目的: 研究纳米雄黄脂质体的制备方法并对其特性进行检测.方法: 采用化学沉淀法先制备纳米级雄黄,薄膜分散-高压均质法制备纳米雄黄脂质体.用电子显微镜、能谱仪、原子荧光光谱仪、激光粒度分析仪对其进行特性研究,并研究其体外抗肿瘤细胞的能力.结果: 薄膜分散-高压均质法制备的雄黄纳米脂质体平均粒径为102.3 nm,分散性良好,球形或卵球形.能谱仪证实其中含有砷和硫的成分,脂质体的药物包封率为82.78%.体外作用于HepG2肝癌细胞显示其有良好的抑制生长作用.结论: 采用上述方法可以成功制备纳米级别的雄黄脂质体,粒径较小,符合纳米制剂的要求,稳定性良好,药物包封率高,并且具备良好的抗肿瘤细胞的作用.纳米雄黄脂质体是一种新型纳米级剂型,为传统中药在抗肿瘤方面的应用提供了新的思路.
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| 关 键 词： | 纳米脂质体 雄黄 特性 抗肿瘤作用 |
Preparation and characterization of realgar nano-liposome and its antitumor effect in vitro |
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| WANG Zi-yu,WANG Li,ZHANG Dong-sheng.Preparation and characterization of realgar nano-liposome and its antitumor effect in vitro[J].Journal of Southeast Univ: Medical Sci Ed,2009,28(3):175-179. |
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| Authors: | WANG Zi-yu WANG Li ZHANG Dong-sheng |
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| Institution: | 1.Department of Pathology;Nanjing University of Traditional Chinese Medicine;Nanjing 210046;China;2.School of Clinical Medicine;Southeast University;Nanjing 210009;3.Department of Pathology and Pathophysiology;School of Basic Medical Science;China |
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| Abstract: | Objective To explore the preparation method and characteristics of realgar nano-liposome and its antitumor effect on liver cancer cells.Methods Realgar nanoparticles were prepared by chemical precipitation method and then realgar nano-liposomes were prepared by film dispersion and high pressure homogenizer method.TEM,EDS,ZETA-SIZER and atom fluorescence spectrophotometer were used to characterize realgar nano-liposomes.MTT assay was performed to examine the antitumor effect of realgar nano-liposomes at vari... |
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| Keywords: | nano-liposomes realgar characterization antitumor effect |
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