| 用改良的盐洗法与酚-氯仿法提取基因组DNA效果比较 |
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| 引用本文： | 胡敬富,周淑华,韩兆东,魏义花.用改良的盐洗法与酚-氯仿法提取基因组DNA效果比较[J].滨州医学院学报,2004,27(2):83-84. |
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| 作者姓名： | 胡敬富 周淑华 韩兆东 魏义花 |
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| 作者单位： | 滨州医学院附属医院,滨州市,256603;滨州医学院附属医院,滨州市,256603;滨州医学院附属医院,滨州市,256603;滨州医学院附属医院,滨州市,256603 |
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| 摘 要： | 目的:探索更为安全可靠的基因组DNA提取方法.方法:采用改良盐洗法提取人外周血白细胞基因组DNA,与传统的酚-氯仿抽提法在提取效果、DNA纯度等方面加以比较.结果:通过对15份不同静脉血标本与酚-氯仿抽提法同步对比研究显示:改良盐洗法提取模板DNA无污染、无害,所提DNA质量好、纯度高,达到酚-氯仿抽提法所获DNA的水平;操作简单、快速,方法稳定可靠,无一例失败.结论:该方法提取的DNA可用于实验以及临床上DNA分型等研究.
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| 关 键 词： | DNA 提取法 盐洗 酚-氯仿 |
| 文章编号： | 1001-9510(2004)02-0083-02 |
| 修稿时间： | 2003年10月21日 |
ON THE EFFECTIVENESS OF IMPROVED SALTING OUT METHOD AND PHENOL-CHLOROFORM ONE FOR EXTRACTION OF GENOME DNA |
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| Hu Jingfu,Zhou Shuhua,Han Zhaodong,et al.ON THE EFFECTIVENESS OF IMPROVED SALTING OUT METHOD AND PHENOL-CHLOROFORM ONE FOR EXTRACTION OF GENOME DNA[J].Journal of Binzhou Medical College,2004,27(2):83-84. |
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| Authors: | Hu Jingfu Zhou Shuhua Han Zhaodong |
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| Abstract: | Objective:To explore a more effective and safe genome DNA extracting method.Methods:An improved salting out method was used to extract human leucocyte genome DNA.This method was compared with traditional phenol chloroform extraction one in 15 different blood samples.Results:The results showed that the salting out method was a simple,rapid and nontoxic extraction procedure.DNA quality obtained by this technique was comparable to that by phenol chloroform extraction.All the 15 tests were done successfully.Conclusion:DNA extracted with this method can be used in such laboratory and clinical research as DNA typing. |
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| Keywords: | DNA extraction salting out phenol chloroform |
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