HPLC法对不同蒲地蓝消炎制剂中绿原酸和咖啡酸含量的测定

目的建立高效液相色谱(HPLC)法同时测定蒲地蓝消炎片、蒲地蓝消炎胶囊和蒲地蓝消炎口服液中绿原酸和咖啡酸的含量。方法以75%甲醇为溶剂,采用超声法提取上述3种不同蒲地蓝剂型中绿原酸和咖啡酸;使用高效液相色谱法测定其含量,色谱柱为waters SunfireTMC18(5μm,4.6×150 mm),流动相为乙腈-0.4%磷酸水溶液(9∶91),流速为1.0 mL.min-1,检测波长为327 nm,柱温为室温。结果绿原酸的检测浓度在14.7～3750.0 ng.mL-1范围内与峰面积积分值呈良好线性关系(r2=0.9974),平均回收率为101.6%,RSD=1.3%;咖啡酸在68.4～4376.0 ng.mL-1范围内与峰面积积分值呈良好线性关系(r2=0.9984),平均回收率为101.1%,RSD=1.3%。结论本法灵敏、简便、准确,可用于不同剂型蒲地蓝消炎制剂中绿原酸和咖啡酸含量测定和质量控制;不同剂型蒲地蓝消炎制剂中绿原酸和咖啡酸含量不一致,应该尽快建立其质量标准。

Content Determination of Chlorogenic Acid and Caffeic Acid in Pudilanxiaoyan Different Preparations by HPLC

Objective To establish a HPLC method for the content determination of chlorogenic acid and caffeic acid in 3 kinds of dosage forms of Pudilanxiaoyan preparation.Methods With methanol-water(75∶25) as the extraction solvent for free chlorogenic acid and caffeic acid,ultrasonic method was used for the extraction.Chlorogenic acid and caffeic acid were separated on a waters SunfireTMC18 column(5 μm,4.6×150 mm) and the mobile phase consisted of acetonitrile-0.4% phosphoric acid(9∶91) at the flow rate of 1.0 mL·min-1.Column temperature was set at room temperature and the detection wavelength was set at 327 nm.Results Chlorogenic acid and caffeic acid were linear within the range of 14.7~3750.0 ng·mL-1(r2=0.9974),68.4~4376.0 ng·mL-1(r2=0.9984),respectively.The average recovery were 101.6%(RSD=1.3%),101.1%(RSD=1.3%).Conclusion The method is sensitive,simple and accurate for the quality control and content determination of 3 kinds of Pudilanxiaoyan preparations.The content of chlorogenic acid and caffeic acid differed greatly in different Pudilanxiaoyan preparations;therefore it is urgent to establish the quality standard for Pudilanxiaoyan preparations as soon as possible.