脂多糖结合熏烟法和单纯熏烟法建立慢性阻塞性肺病大鼠模型的比较

目的比较熏香烟加气道内注入脂多糖(LPS)法和单纯熏香烟法建立慢性阻塞性肺病(COPD)大鼠模型的效果。方法8周龄Wistar大鼠24只,随机分为3组,每组8只。其中1组作健康对照,另2组分别进行熏香烟加气道内注入LPS和单纯熏香烟处理建立COPD模型。观察动物一般情况和肺组织病理学,测定肺组织平均内衬间隔(MLI)和平均肺泡数(MAN);检测外周血常规和支气管肺泡灌洗液(BALF)常规。结果两个模型组大鼠消瘦,伴有间歇咳嗽和气促,外周血和BALF中的白细胞总数及中性粒细胞百分比均较对照组明显增高(P<0.01);肺组织H-E染色显示两个模型组大鼠均具有慢性支气管炎和肺气肿的典型病变,MLI较对照组明显增高,而MAN较对照组明显下降(P<0.01),但两个模型组组间差异无统计学意义;熏香烟加气道内注入LPS组比单纯熏香烟组气道及肺组织的炎症浸润更明显,单纯熏香烟组主要表现为肺泡过度扩张。结论熏香烟加气道内注入LPS和单纯熏香烟两种方法均可成功制备大鼠COPD模型,其病理生理改变与人类COPD类似,前者比后者更符合COPD自然发病过程。

Establishment of rat chronic obstructive pulmonary disease model: a comparison between exposure to cigarette smoke alone and in combination with intra-tracheal injection of lipopolysaccharide

Objective To compare the efficacies of exposure to cigarette smoke alone and in combination with intra-tracheal injection of lipopolysaccharide in establishing rat models of chronic obstructive pulmonary disease(COPD).Methods A total of 24 8-week-old healthy Wistar rats were randomly divided into 2 model groups and a control group(Group C).The rat COPD models were established by two ways:intratracheal injection of lipopolysaccharide(LPS) twice + exposure to cigarette smoke for 1 month(Group A),and cigarette...