慢性乙型重型肝炎患者外周血各亚群淋巴细胞绝对值的特点

目的:分析慢性乙型重型肝炎(以下简称慢重肝)患者外周血各亚群淋巴细胞绝对值的特点.方法:慢重肝患者61例,乙肝引起的肝硬化26例,慢性乙型肝炎21例,健康志愿者10例.采用流式细胞仪检测各自外周血淋巴细胞CD3 、CD4 、CD8 、NK细胞(CD3-/CD16 /CD56 )、NKT细胞(CD3 /CD16 CD56 )等亚群表达百分比,计算各淋巴细胞亚群绝对值,并进行统计学分析.结果:与乙肝引起的肝硬化、慢性乙型肝炎患者以及健康志愿者相比,慢重肝患者CD3 、CD4 及CD8 T淋巴细胞的绝对值均明显降低(P＜0.01或P＜0.05);与存活者相比,慢重肝死亡患者外周血淋巴细胞、CD3 、CD4 、CD8 、NK细胞、NKT细胞绝对值均有一种降低的趋势但尚未达到统计学意义.结论:慢重肝患者存在一定程度的细胞免疫功能紊乱,可能与其预后存在一定的相关性.

Characteristics of peripheral circulating lymphocyte subsets and their clinical implication in severe hepatitis patients with chronic HBV infection

Objective:To investigate the characteristics of peripheral circulating lymphocyte subsets in severe hepatitis patients with chronic HBV infection(SHPCHI).Methods: The numbers of circulating lymphocyte subsets,including CD3~(+),CD4~(+) and CD8~(+),NK cells(CD3~(-)/CD16~(+)/CD56~(+)),and NKT cells(CD3~(+)/CD16~(+)CD56~(+)),were determined in SHPCHI((n=61),)patients with chronic hepatitis B(CHB,n=21),patients with cirrhosis(LC,n=26) and healthy volunteers(n=10) by flow cytometry.The absolute values of each lymphocyte subset were calculated and statistically analyzed.Results: The absolute numbers of circulating CD3~(+),CD4~(+)and CD8~(+) T cells were significantly reduced in SHPCHI group compared with those in the other 3 groups(P<0.01 or P<(0.05).) There was a decreasing trend in the absolute numbers of peripheral CD3~(+),CD4~(+),CD8~(+) and NK cells in non-surviving SHPCHI compared with surviving SHPCHI,but the decrease was not statistically significant.Conclusion: SHPCHI has an disorderd cellular immune function,which might be related to the prognosis of SHPCHI.