良恶性脑瘤患者手术前后免疫功能的变化

动态观察良恶性脑瘤患手术前后的外周血自然杀细胞活性，淋巴因子活化的杀伤细胞活性及Ｔ细胞亚群的变化。方法：良恶性脑瘤患分为两组，采用ＡＢＣ免疫组化法测定Ｔ淋巴细胞亚群的用＾３Ｈ－ＴｄＲ标记靶细胞杀伤释放法测定ＮＫ，ＬＡＫ活性。结果；两组患术后第３天的ＮＫ活性，ＬＡＫ活性的ＣＤ＾＋３，ＣＤ＾＋４细胞及ＣＤ＾＋４／ＣＤ＾＋８比值明显低于术前，ＣＤ＾＋８细胞则明显高于术前，相差均非常显，术后第７天

Changes of immune function in patients with brain tumors in perioperation

Objective: To observe the dynamic changes of natural killer cell (NK),lymphokine activated killer cells (LAK) activity and lymphocyte subpopulation for the patients with malignant and benign brain tumor in perioperation period. Methods: The patients were divided into two groups. Lymphocyte subsets were assayed by ABC immuno histochemistry. NK and LAK activities were detected by 3H TdR incorporation target cell release method. Results: NK,LAK activity and the quantitation of CD3 +,CD4 + subpopulation and the CD4 +/CD8 + ratio 3 d after operation were significantly lower than those of preoperation, however, the percentage of CD8 + was higher than that of preoperation in both groups (P<0.01). The NK,LAK cells activity and the quantitation of lymphocyte subpopulation were gradually increased 7 d after operation in both groups. Besides the percentage of CD8 + and CD4 +/CD8 + ratio in 10 d after operation had significant difference from those of preoperation. LAK cells activity recovered gradually by 10 d after operation in both groups, other quantitations were access to those of preoperation. Conclusion: Immune function in patients with brain tumor is recovered to different degree in different period after operation, providing references to clinical therapy.