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| 尼古丁对小鼠巨噬细胞凋亡的影响 | |
| 引用本文： | 王昌惠,刘忠令,李强,李莉,钟晓松.尼古丁对小鼠巨噬细胞凋亡的影响[J].第二军医大学学报,1999,20(12):974-976,F004. |
| 作者姓名： | 王昌惠 刘忠令 李强 李莉 钟晓松 |
| 作者单位： | 1. 第二军医大学长海医院呼吸内科,上海,200433 2. 第二医大学长征医院检验科 3. 上海肿瘤研究所 |
| 基金项目： | 国家自然科学基金!批准号 3 790 0 0 6 6 |
| 摘 要： | 目的：研究尼古丁对小鼠巨噬细胞（Ｍψ）凋亡的影响。方法：用不同浓度（１０＾－８￣１０＾－２ｍｏｌ／Ｌ）的尼古丁与小鼠腹腔Ｍψ体外共同培养，测定Ｍψ存活率；采用荧光和流式细胞术观察Ｍψ凋亡的发生情况。结果：一定浓度（１０＾－４￣１０＾－８ｍｏｌ／Ｌ）的尼古丁对Ｍψ凋亡具有抑制作用。结论：尼古丁可能通过抑制Ｍψ凋亡在肺气肿的发病中起重要作用。 |
| 关 键 词： | 巨噬细胞 凋亡 尼古丁 |
| 文章编号： | 0258-879X(1999)12-0974-03 |
Nicotine prolongs the survival of rat macrophages by inhibiting its apoptosis |
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| Wang Changhui,Liu Zhongling,Li Qiang,Li Li,Zhong Xiaosong.Nicotine prolongs the survival of rat macrophages by inhibiting its apoptosis[J].Academic Journal of Second Military Medical University,1999,20(12):974-976,F004. | |
| Authors: | Wang Changhui Liu Zhongling Li Qiang Li Li Zhong Xiaosong |
| Abstract: | Objective: To study the effect of nicotine on the rat peritoneal macrophages. Methods:The survival rate of macrophages with or without nicotine in vitro was studied, and the nicotine effect on macrophages apoptosis at different concentration by using fluorescence dye and flow cytometry was analyzed. Results: The survival rate of peritoneal macrophages cultured with nicotine was higher than without nicotine. The best concentration of nicotine is 10 -6 mol/L. The prolonged survival rate of macrophages was due to the inhibition of macrophages apoptosis. Conclusion: Cigarette smoking may cause abnormal apoptosis of alveolar macrophages which play an important role in emphysema. |
| Keywords: | macrophages apoptosis nicotine |
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