细胞周期蛋白D1基因表达在心肌肥大形成中的时相性变化

目的：观察心肌肥大形成过程中细胞周期蛋白Ｄ１（ｃｙｃｌｉｎ Ｄ１）基因表达的时相性变化，并探讨ｃｙｃｌｉｎ Ｄ１在心肌肥大形成中的作用。方法：肛用腹主动脉缩窄法建立大鼠心肌肥大模型，设缩窄手术组及假手术对照组，观察两组大鼠手术后不同时间心脏左心室（含左心室游离与室间隔）质量与体质量比、左心室游离壁厚度及心肌细胞横径的变化，以确证模型的稳定建立；其后应用ＲＮＡ印迹法分析检测ｃｙｃｌｉｎ Ｄ１基因表达

Expression of cyclin D1 gene during the development of myocardial hypertrophy

Objective: To observe the expression of cyclin D 1 gene during the development of myocardial hypertrophy and investigate the effect of cyclin D 1 on formation of myocardial hypertrophy. Methods: The experimental myocardial hypertrophy rat model was constructed through abdominal aortic banding, and was proved to be efficient by pathologic detection including ratio of left ventricular(LV) mass/body weight, thickness of LV disassociated wall and myocytes width in stenosis and control groups. The mRNA level of cyclin D 1 of rat myocardium was analyzed by Northern blotting. Results: There was significant increase of LV mass/body weight, thickness of LV disassociated wall and myocytes width in stenosis group 4 but not 2 weeks after constriction ( P <0.05). Cyclin D 1 mRNA level increased greatly in stenosis group both 2 and 4 weeks after constriction( P <0.05). Conclusion: Cylin D 1 mRNA level increased significantly during the development of myocardial hypertrophy before pathologic change suggest the possible role of cyclin D 1 in the formation of myocardial hypertrophy.