苦参碱、氧化苦参碱和槐定碱对巨噬细胞RAW264.7表达CD91、CD13和分泌TNF α的抑制作用

目的探讨苦参碱、氧化苦参碱和槐定碱对巨噬细胞RAW264.7表达CD91、CD13和分泌TNF-α的抑制作用。方法(1)用6孔板培养巨噬细胞RAW264.7,制作细胞爬片,然后与不同浓度(0、12.5、25、50、100、200mg/L)的苦参碱、氧化苦参碱和槐定碱共培养,细胞爬片免疫组化染色,检测CD91和CD13的表达情况,并采用MediaCybernetics公司的图像分析系统进行定量分析,测量积分光密度(IOD)值。(2)6孔培养板培养巨噬细胞RAW264.7,加入不同浓度的苦参碱、氧化苦参碱和槐定碱与巨噬细胞共培养1d后,收集培养孔中的培养液,采用ELISA方法检测培养液中TNF-α的含量。结果苦参碱、氧化苦参碱和槐定碱均能抑制巨噬细胞表达CD91和CD13,其抑制作用随浓度变化也有不同,苦参碱更明显。不同浓度的苦参碱、氧化苦参碱和槐定碱对巨噬细胞分泌TNF-α有抑制作用,苦参碱对巨噬细胞RAW264.7分泌TNF-α的抑制作用更明显。结论苦参碱、氧化苦参碱和槐定碱对巨噬细胞表达CD91、CD13和分泌TNF-α有明显的抑制作用。

Matrine, oxymatrine and sophordine inhibit secretion of TNF-α and expression of CD91 and CD13 in macrophage RAW264. 7

Objective To observe the inhibitory effects of matrine, oxymatrine and sophordine on expression of CD91 and CD13 and secretion of TNF-α in macrophage RAW264.7. Methods Macrophages were cultured in 6-hole plate and coverslip was prepared. Then macropages were cultured one day with different concentrations(0,12.5,25,50,100,200 mg/L) of matrine, oxymatrine and sophordine; the expression of CD91 and CD13 by immunohistochemical dyeing was analyzed by Image-Pro plus system (Media Cybernetic, Inc. , 6.0 edition). Meanwhile, the levels of TNF-α in the culture medium of maerophages were examined by ELISA. Results Matrine, oxymatrine and sophordine could all inhibit CD91 and CD13 expression in macrophage RAW264.7, and the inhibition varied with the concentrations of matrine, oxymatrine and sophordine; the inhibitory effect of matrine was the most prominent one. Moreover, matrine, oxymatrine and sophordine also inhibited the secretion of TNF-α.Conclusion Matrine, oxymatrine and sophordine can greatly inhibit expression of CD91 and CD13 and secretion of TNF-α in macrophage RAW264.7.