氧化苦参碱及甘草甜素对小鼠肝细胞凋亡的影响

目的：探讨氧化苦参碱(OM)及甘草甜素(GL)对撤除苯巴比妥钠(PB)诱导的小鼠肝细胞凋亡的影响。方法：选用昆明种小鼠，测定肝/体质量比、肝组织DNA含量，观察组织学改变及原位凋亡细胞TUNEL标记等指标，观察腹腔内注射OM 150mg/kg及GL 50mg/kg，3次/d，于36h后，对撤除PB后引起的小鼠肝细胞凋亡的影响。结果：阳性对照组肝/体质量比及肝DNA含量分别回落16.3％及32.4％；GL组分别回落16.1％及28.9％；OM组无明显回落。组织学检查及凋亡细胞TUNEL标记显示：阳性对照组及GL组小鼠肝细胞出现典型凋亡改变，TUNEL标记阳性；阴性对照组及OM组小鼠肝细胞未见明显凋亡改变。结论：OM可阻断撤除PB诱导的小鼠肝细胞凋亡，GL对其无影响。

Effect of oxymatrin and glycyrrhizin on hepatocyte apoptosis induced by cessation of phenobarbital treatment in mice

Objective: To investigate the effects of oxymatrin (OM) and glycyrrhizin (GL) on hepatocyte apoptosis induced by cessation of phenobarbital(PB) treatment in mice. Methods: The Kunming mice were treated with OM (150mg/kg,ip) and GL (50mg/kg,ip) 3times daily during 36h after cessation of PB treatment. Hepatic DNA content and the ratio of liver/body mass of mice were observed to estimate the regressive rate of hyperplastic liver at 36h after withdrawing PB. Histomorphology and the end-labeling method termed TUNEL (TdT-mediated X-dUTP nick end labeling) were used to identify apoptotic cells and DNA in apoptotic cells. Results: Regression ratio of hepatic DNA content and liver/body mass in the positive control group was 32.4%and 16.3%, and 28.9%and 16.1%in the group treated with GL. Hepatic DNA content and liver/body mass in the group treated with OM produced little regression as compared with the negative control group. The results showed that OM markedly inhibited the regression of hepatic DNA content and liver/body mass, but GL showed no effect. Additionally, those mice in the positive control group and the group treated with GL produced a typical hepatocyte apoptotic change with a positive result of TUNEL assay. No hepatocyte apoptotic changes were found in the liver of mice treated with OM, and a negative result of TUNEL assay was presented. Conclusion: OM inhibits the regression of hepatic DNA content and liver/body mass by preventing hepatocyte apoptosis induced by cessation of PB treatment in mice.