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检测乙、丙型肝炎病毒基因芯片的制备
引用本文:吴海,顾少华,应康,谢毅,毛裕民,胡志前,许一斌,徐东.检测乙、丙型肝炎病毒基因芯片的制备[J].第二军医大学学报,2001,22(6):527-529,T003.
作者姓名:吴海  顾少华  应康  谢毅  毛裕民  胡志前  许一斌  徐东
作者单位:1. 复旦大学
2. 第二军医大学长征医院普通外科
3. 联合基因上海博华芯片技术有限公司
基金项目:国家自然科学基金资助项目 (3 0 0 0 0 0 46)
摘    要:目的:探讨研制检测乙型和丙型肝炎病毒的基因芯片。方法:以HBV、HCV高度保守的基因片段为探针,同时用没有同源性的植物基因片段为内参照基因,制备成“乙、丙型肝炎病毒双检基因芯片”。检测时抽提患者血清中的病毒核酸,进行RT-PCR扩增及荧光标记,然后与双检基因芯片杂交,结交结果用ScanArray3000扫描仪扫描。结果:双检基因芯片的内参照结果能做到绝对阳性和绝对阴性,同时对不同血清(正常人血清,乙肝E抗原阳性血清,丙肝RNA阳性血清,乙肝、丙肝阳性混合血清)能有效检测区分。结论:本研究成功地制作了乙、丙型肝炎病毒双检基因芯片,可望使用于临床。

关 键 词:基因芯片  基因诊断  乙型肝炎病毒  丙型肝炎病毒
文章编号:0258-879X(2001)06-0527-03

Development of diagnostic microarray in detection of HBV and HCV
WU Hai ,GU Shao Hua ,HU Zhi Qian ,XU Yi Bin ,XU Dong ,YING Kang ,XIE Yi ,Mao Yu Min.Development of diagnostic microarray in detection of HBV and HCV[J].Academic Journal of Second Military Medical University,2001,22(6):527-529,T003.
Authors:WU Hai  GU Shao Hua  HU Zhi Qian  XU Yi Bin  XU Dong  YING Kang  XIE Yi  Mao Yu Min
Institution:WU Hai 1,GU Shao Hua 1,HU Zhi Qian 2,XU Yi Bin 3,XU Dong 3,YING Kang 1,XIE Yi 1*,Mao Yu Min 1*
Abstract:Objective: To investigate the value of diagnosis microarray in detection of hepatitis B and C virus. Methods: The conservative sequences of hepatitis B and C virus were used as probe. The non homology sequences of vegetative genes were used as control. When it was used, the patient serum was abstracted. The viral nucleotides were reversely transcribed and enlarged by polymerase chain reaction (PCR). Then it was labeled with fluorescence, and hybridized with the diagnosis genechip. The genechips were scanned using scanner (ScanArray 3000). Results: The diagnosis genechip included positive and negative controls. All kinds of serum (normal, E antigen positive, HCV RNA positive, mixed serum of HBV and HCV) could be detected. Conclusion: Diagnosis genechip for HBV and HCV can be used for clinic diagnosis after operators are trained.
Keywords:DNA microarray  gene diagnosis  hepatitis B virus  hepatitis C virus
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