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人肾细胞癌细胞的原代培养及癌基因表达初步分析
引用本文:戚中田,侯建国,曹广文,张晓琴.人肾细胞癌细胞的原代培养及癌基因表达初步分析[J].第二军医大学学报,1999,20(5):280-282.
作者姓名:戚中田  侯建国  曹广文  张晓琴
作者单位:1. 第二军医大学基础医学部微生物学教研室,上海,200433
2. 第二军医大学长海医院泌尿外科
摘    要:目的:研究人肾细胞癌(RCC)原代细胞生物学特性及其癌基因的表达情况。方法:应用改良的原代肿瘤细胞分离法分离培养7例人RCC原代细胞。应用染色体核型分析和免疫组化方法,分析原代细胞核型以及癌基因与抑癌基因的表达。结果:用本法培养的原代细胞比其他常规方法所得细胞既多且纯。所培养的人RCC原代细胞的癌基因表达分析发现bcl-2,c-erbB-2,c-met,表皮生长因子受体(EGFR)有轻度至中度表达,而p53没有表达。结论:人RCC原代培养及其癌基因表达检测,为应用原代RCC所开展exvivo的RCC基因治疗打下了初步的基础。

关 键 词:肾肿瘤  原代培养  癌基因

Preliminary analysis of primarily cultured cells of human renal cell carcinoma and expression of the oncogenes
Zhang Xiaoqin,Cao Guangwen,Hou Jianguo,Qi Zhongtian.Preliminary analysis of primarily cultured cells of human renal cell carcinoma and expression of the oncogenes[J].Academic Journal of Second Military Medical University,1999,20(5):280-282.
Authors:Zhang Xiaoqin  Cao Guangwen  Hou Jianguo  Qi Zhongtian
Abstract:Objective: To study the characteristic of primary cells of human renal cell carcinoma(RCC) and expression of the oncogenes. Methods: An improved isolation method was employed to isolate primary RCC cells from 7 fresh human RCC specimens. The expression of some oncogenes in the cells was examined by means of chromosomal pattern analysis and immunohistochemistry. Results:It was found that the primary cells obtained by using this met hod was more and purer than that of the routine methods and bcl-2, c-erbB-2 , c-met, EGFR had low to medium level expression, but p53 had no expression. Conclusion: The study provides a basis for the ex vivo gene th erapy for RCC.
Keywords:kidney neoplasms  primary cell culture  oncogenes
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