人神经干细胞临床移植治疗帕金森病

目的:探索人神经干细胞移植治疗帕金森病(Parkinson disease,PD)的有效方法.方法:5～11周人胚胎的前脑细胞在体外稳定扩增,并向多巴胺神经元转化,通过立体定向手术将其植入PD患者的纹状体;2000年11月至2002年11月,采用此方法治疗50例PD患者.结果:人胚胎前脑细胞可在体外扩增并维持不分化状态1年以上,扩增的细胞可达原始细胞数量的1×107倍,并可向多巴胺能神经元转化;PD患者临床移植手术后随访8～30个月(平均24个月),有效率为92％,未见明显的免疫排斥反应.结论:应用体外长期扩增的人类神经干细胞移植治疗PD是可行和有效的.

Clinical transplantation of human neural stem cells in treatment of Parkinson disease

Objective:To study the clinical effect of transplanting human neural stem cells in treatment of Parkinson disease (PD). Methods: The cells from human fetal forebrain (5 11 weeks gestation) were expanded in vitro and differentiated into dopamine neurons, which were then implanted in PD striatum with stereotactic operation in 50 PD patients from Nov. 2000 to Nov. 2002. Results: The cells of fetal forebrain could be expanded by 10 7 fold in undifferentiated state for more than one year, and they could be turned into dopamine neurons. After 8 30 months (averagely 24 months) follow up,the effective rate of operation was 92%. Conclusion: It is feasible to implant the neural stem cells expanded in vitro for long period in treating PD.