清醒大鼠冠状动脉阻断再通技术及其应用

目的；设计冠状动脉阻断与再通控制技术，用于建立清醒自由活动大鼠心肌缺血再灌注模型。方法；大鼠麻醉开胸后，左冠状支脉牵拉线穿过胸内一段小塑料管，然后引出体外。清醒状态下，牵拉该线时，可阻断冠状动脉，放松后使冠状动脉再通。结果；心肌缺血３０ｍｉｎ内，清晰大鼠１６（１６／２５）只存活，而麻醉开胸大鼠９（９／１０）只存活。利多卡因使清晰大鼠缺血性室颤降至８．３％（２／２４），而不影响心肌梗塞范围。清晰大

Technique for coronary occlusion-reperfusion and its application in conscious rats

Objective:To develop a technique for coronary occlusion-reperfusion,which may be used to establish a model of myocardial ischemia and reperfusion in the uncontrolled conscious rat. Methods : After the rat had been anesthetized and thoracotomized,a silk thread for pulling the left coronary artery was passed through a plastic tube inside the thoracic cavity and then exteriorized.The coronary artery in the conscious rat could be occluded and reperfused while the thread was pulled and released respectively.Results:During 30 min of myocardial ischemia, 16(16/25) conscious rats and 9(9/10) anesthetized and open-chest rats survived.The incidence of ischemic ventricular fibrillation was decreased to 8.3%(2/24) by lidocaine given before ischemia, but myocardial infarction size was not affected. The heart rate during ischemia in conscious rats was more markedly rapid than anesthetized rats, and demonstrated a peak value at 5 min of ischemia. ST segments of 14(14/16) rats in the control group and 21 (21/22) rats in the lidocaine group decreased suddenly when the pulling thread was released following 30 min of coronary occlusion.Our method had a higher reperfusion success rate (14/16) than Himori method (6/14). Conclusion: A simple and reliable technique for coronary occlusion and reperfusion in uncontrolled conscious rats has been developed ,which may result in a higher success rate and is valuable for further application.