变应性鼻炎鼻粘膜中一氧化氮合酶的组织化学变化

目的：通过比较豚鼠正常和变应性鼻炎（ＡＲ）鼻粘膜中一氧化氮合酶（ＮＯＳ）的分布，探讨ＮＯＳ与ＡＲ的关系。方法：应用酶组织化学技术，以还原型尼克酰胺腺嘌呤二核苷酸磷酸－黄递酶（ＮＡＤＰＨ－ｄ）特异性地测定ＮＯＳ在正常组和ＡＲ组豚鼠鼻粘膜中的分布及ＡＲ组ＮＯＳ活性变化。结果：正常和ＡＲ组豚鼠鼻粘膜酶组织化学染色均有反应，ＡＲ组呈强阳性反应，主要分布于腺体细胞、上皮细胞及血管内皮细胞胞质。结论：正常豚鼠

Histochemical changes of nitric oxide synthase in nasal mucosa of allergic rhinitis

Objective: The activity of nitric oxide synthase(NOS)in nasal mucosa of normal guinea pigs was compared with that of allergic rhinitis(AR)guinea pigs to investigate the relationship between NOS and AR. Methods: Localization of NOS in nasal mucosa of normal and AR guinea pigs was examined by means of NADPH diaphorase (NADPH d) histochemical stain. Results: Nasal mucosa of both normal and AR groups was positive for NADPH d, though the AR group reacted more strongly. Reaction was localized to the cytoplasm of glandular cell, epithelium and vascular endothelium. Conclusion: There is distribution of NOS in nasal mucosa of normal guinea pigs. The activity of NOS in guinea pig AR model was significantly stronger than that in normal group. NOS is related to AR.