| PBR322:RSF1010重组质粒的构建 |
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| 引用本文： | 温肇荣.PBR322:RSF1010重组质粒的构建[J].第二军医大学学报,1984(4). |
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| 作者姓名： | 温肇荣 |
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| 作者单位： | 第二军医大学流行病学教研室 |
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| 摘 要： | 分别从大肠杆菌HB802(pBR322)及C600CRSF1010菌株中抽提质粒,用EcoRI酶切后,再用T_4DNA连接酶将它们连接,转化大肠杆菌C_(600)菌株,共获得253株转化子。经琼脂糖凝胶电泳及电镜摄影证实质粒PSMMI是pBR322:RSF1010重组体,可使宿主菌C_(600)获得对ApTc及Sm的抗性。本文对重组体pSMMI中pBR322和RSF1010的连接方向及这一重组质粒未能转化恶臭杆菌AC_(10)菌株的原因,进行了讨论。
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| 关 键 词： | 质粒 载体 转化 遗传 重组 遗传 |
THE CONSTRUCTION OF HYBRID pBR322: RSF1010 |
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| Wen Zhaorong.THE CONSTRUCTION OF HYBRID pBR322: RSF1010[J].Academic Journal of Second Military Medical University,1984(4). |
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| Authors: | Wen Zhaorong |
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| Institution: | Wen Zhaorong Department of Epidermiology |
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| Abstract: | The plasmids pBR322 and RSF1010, extracted from E.coli HB802 and E. coli C600 respectively were treated with restrictive endonuclease EcoRI and ligated with T4 DNA ligase. C600 strains were then transformed with the hybrid plasmid, and thus 253 transformants were obtained.The figures of gel elctrophoresis and electromicrograph proved that PSMM1 was a pBR322 : RSF1010 composite hybrid.In E. coli C600 PSMM1 obtained resistance against AP, Tc and Sm.The orientation of pBR322 and RSF1010 in hybrid PSMM1 and the failure of introduction of this hybrid into P.Putida AC10 were discussed |
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| Keywords: | Plasmids cloning vector transformation genetic recombination genetic |
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