乙肝核心抗原DNA疫苗的构建及其免疫原性分析

构建ＨＢＶ核心抗原ＤＮＡ疫苗，并探讨基实验动物内的表达及其免疫原性。方法：采用ＰＣＲ法从ＨＢＶ全基因ＤＮＡ中获得核心抗原ＤＮＡ片段，并将其重组到ｐｃＤＮＡ３载体。以此为候选疫苗分别免疫小鼠和树Ｊｕ，并检测其抗ＨＢｃＩｇＧ。结果：构建了乙肝核心抗原候选核酸疫苗ｐｃＤＮＡ３－ＨＢｃ。免疫接种该疫苗后第２周抗ＨＢｃＩｇＧ水平开始升高，小鼠至第９周，树Ｊｕ至第７周升至峰值。结论：构建ｐｃＤＮＡ３－ＨＢＣｄ

Construction of hepatitis B virus core antigen DNA vaccine and its immunogenic analysis

Objective: To construct DNA vaccine for HBV core antigen and study its expression and immunogenicity in animals. Methods:HBc DNA fragment was obtained from HBV whole genomic DNA with PCR and was cloned into DNA vaccine vector pcDNA3. The constructed candidate for DNA vaccine was immunized in mice and tree shrew and it's anti HBc IgG was detected. Results: A recombinant DNA vaccine pcDNA3 HBc was constructed. Anti HBc IgG was detected 2 weeks after immunization and continued to increase until week 9 in mice and week 7 in tree shrew. Conclusion: DNA vaccine pcDNA3 HBc is expressed in vivo and induces humoral response.