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利用稻瘟霉分生孢子筛选有生物活性的海洋生物
引用本文:汤海峰,易杨华,姚新生,林厚文,张淑瑜,吴久鸿.利用稻瘟霉分生孢子筛选有生物活性的海洋生物[J].第二军医大学学报,2002,23(3):246-249.
作者姓名:汤海峰  易杨华  姚新生  林厚文  张淑瑜  吴久鸿
作者单位:1. 第二军医大学药学院海洋药物研究中心,上海,200433
2. 沈阳药科大学中药系
3. 第二军医大学长征医院药学部
4. 解放军第306医院
基金项目:国家自然科学基金资助项目 ( 30 0 0 0 2 19)
摘    要:目的:筛选有生物活性的海洋生物并研究其活性成分.方法:采用稻瘟霉模型筛选海洋生物提取物的活性,利用多种层析手段和波谱技术对活性提取物跟踪分离并鉴定其成分.结果:实验发现20种海洋生物醇提物和5种水提物对稻瘟霉显示生物活性,从叶托马尾藻、铁钉菜和蓝斑背肛海兔中分离鉴定了56个化合物,其中28个对稻瘟霉显示活性且对不同肿瘤细胞株显示不同强度的细胞毒性,8个化合物对裴氏着色真菌具抑制作用.结论:利用稻瘟霉模型对海洋生物的活性成分进行跟踪分离是可行的.

关 键 词:稻瘟霉、叶托马尾藻、铁钉菜、蓝斑背肛海兔、药物筛选
文章编号:0258-879X(2002)03-0246-04
修稿时间:2001年11月12

Using conidia of Pyricularia oryzae screening for bioactive portions from marine organisms
TANG Hai Feng ,YI Yang Hua ,YAO Xin Sheng ,LIN Hou Wen ,ZHANG Shu Yu ,WU Jiu Hong.Using conidia of Pyricularia oryzae screening for bioactive portions from marine organisms[J].Academic Journal of Second Military Medical University,2002,23(3):246-249.
Authors:TANG Hai Feng  YI Yang Hua  YAO Xin Sheng  LIN Hou Wen  ZHANG Shu Yu  WU Jiu Hong
Institution:TANG Hai Feng 1,YI Yang Hua 1,YAO Xin Sheng 2,LIN Hou Wen 3,ZHANG Shu Yu 1,WU Jiu Hong 4
Abstract:Objective:To screen for the bioactive marine organisms and to study the active constituents from them.Methods:Bioactivities of marine organisms extracts were screened by Pyricularia oryzae bioassay method and the constituents were isolated and identified by a combination of multi chromatography and spectral analysis.Results:Twenty ethanolic extracts and 5 aqueous extracts of the tested marine organisms showed activities causing morphological abnormality of P.oryzae mycelia.Fifty six compounds were obtained and identified from Sargassum carpophyllum,Ishige okamurai and Natarchus leachii freeri ,among which 28 compounds showed activities against P.oryzae and exhibited cytotoxicities on various cultured tumor cell lines,eight compounds showed inhibition effects on Fonsecaea pedrosoi in vitro .Conclusion:The screening data and bioactivity evaluation suggest the P.oryzae bioassay is suitable for preliminary screening of bioactive agents from marine organisms.
Keywords:Pyricularia oryzae  Sargassum carpophyllum  Ishige okamurai  Natarchus leachii freeri  drug screening
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