2009年新型甲型H1N1流感病毒神经氨酸酶基因进化分析

目的:探讨2009年新型甲型H1N1流感病毒神经氨酸酶(NA)基因的进化及NA基因编码蛋白抗原性、酶活性位点、糖基化位点变异情况。方法:从NCBI基因库检索获得43株不同年代不同地域甲型流感病毒NA基因序列,用Molecular Evolutionary Genetics Analysis version 4.0（MEGA 4.0）软件进行基因进化分析和氨基酸序列分析。结果:2009年新型甲型H1N1流感病毒与禽H5N1流感病毒NA基因的同源性达到85％,潜在抗原位点氨基酸分布相同;所有毒株的酶活性中心位点高度保守,但糖基化位点有变异。结论:2009年新型甲型H1N1流感病毒的NA基因可能来源于禽H5N1流感病毒;神经氨酸酶抑制剂治疗有效。

Genetic characterization of neuraminidase gene of novel influenza virus A/H1N1 in 2009 pandemic

Objective:To investigate the genetic characterization (evolution,antigenicity,enzymatically active site,and glycosylation site) of the neuraminidase (NA) gene of the novel influenza virus A/H1N1 in 2009 pandemic. Methods:Genetic evolution and amino acid substitutions of the 43 NA gene sequences of influenza virus A of different years were retrieved from GenBank and were analyzed by using MEGA 4.0 software. Results:The new A/H1N1 influenza virus strains and avian H5N1 influenza virus strains had similar antigen sites of the NA genes,sharing a homology of 85%.Amino acid residues at the enzymatically active site of the NA genes were strictly conserved in all influenza virus strains,but the glycosylation sites varied. Conclusion:The NA genes of novel H1N1 influenza viruses may originate from avian H5N1 influenza virus endemic in Asia. The new H1N1 influenza viruses may respond well to the neuraminidase inhibitor treatment.