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部分纯化抗原半定量检测幽门螺杆菌感染
引用本文:路又可 许国铭. 部分纯化抗原半定量检测幽门螺杆菌感染[J]. 第二军医大学学报, 1997, 18(5): 449-451
作者姓名:路又可 许国铭
作者单位:第二军医大学长海医院消化内科
摘    要:鉴定并提取幽门螺杆菌特异性抗原,建立测定抗HP抗体的半定量免疫酶技术。方法:10株HP可溶性蛋白用于十二烷在磺酸钠-聚丙烯酰胺凝胶电泳和蛋白印迹分析,凝胶层析提取特异性抗原,方阵滴定建立免疫酶方法。纯化抗原与全菌抗原同批检测136例患者,以蛋白印迹分析作为金标准。

关 键 词:幽门螺杆菌 蛋白印迹 HP抗体 ELISA

Purification of the specific antigens of Helicobacter pylori for semiquantitative serodiagnosis
Lu Youke,Xu Guoming,Zhou Zhiqun,Zhang Hongfu. Purification of the specific antigens of Helicobacter pylori for semiquantitative serodiagnosis[J]. Former Academic Journal of Second Military Medical University, 1997, 18(5): 449-451
Authors:Lu Youke  Xu Guoming  Zhou Zhiqun  Zhang Hongfu
Abstract:Objective:To extract and analyse the specific antigens of Helicobacter pylori (HP), which are to be used for establishment of a new immunoenzyme technique for anti HP detection. Methods: The soluble proteins from ten HP strains were analysed with SDS PAGE and Western bloting, and a newly elaborated method with square titration was established for detecting anti HP IgG by ELISA. 136 serum samples were examined simultaneously with the specific antigens and whole cell antigens of HP and immunoblot was used as a gold standard. Results: The major immunogenic polypeptides of HP were the bands at 66 000 ,59 000,31 000 and 25 000, out of which three antigens were obtained for detection. The results obtained by the new immunoenzyme methods were a sensitivity of 98.9%, specificity 92.5%, accuracy 97.0 %, respectively. Conclusion: Depurant antigens used for measuring anti HP IgG are superior to whole cell antigen in ELISA.
Keywords:Helicobacter pylori  Western bloting  gel chromatography  enzyme linked immunosorbent assay  
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