肿瘤转移相关基因的表达谱研究

目的：运用基因表达谱芯片技术对肿瘤转移相关基因的表达谱进行研究。方法：对临床切除的肝癌和胰腺癌组织及相应的对照正常组织进行总ＲＮＡ抽提，纯化后的ｍＲＮＡ进行逆转录制备杂交探针，应用ＢｉｏＤｏｏｒ４０９６和ＢｉｏＤｏｏｒ１２８００的全长基因ＤＮＡ芯片筛选与肝癌和胰腺癌转移相关的基因。杂交后的信号用ＳｃａｎＡｒｒａｙ３０００扫描，结果用ＩｍａＧｅｎｅ３．０软件分析。结果：对肝癌和胰腺癌基因表达谱进行分

Study on metastasis-associated gene in carcinoma by cDNA microarray

Objective: To understand the molecular pathophysiology of hepatocellular carcinoma and pancreatic cancer. Methods: We studied novel gene expression by cDNA microarray method. The PCR products of 4 096 genes and 12 800 gene were spotted onto a kind of chemical material coated glass slide in array. Both the mRNAs from 5 cases of hepatocellular carcinoma and 3 cases of pancreatic cancer were reversely transcribed to cDNAs with the incorporation of fluorescent labeled dUTP to prepare the hybridization probes. After hybridization, BioDoor4096 and BioDoor12800 cDNA microarray were scanned for the fluorescent intensity. Tumor invasion related gene expression was screened through the analysis of difference in gene expression profile. Results: Among 4 096 and 12 800 target genes, there were 15 genes whose expression level differed from normal and carcinoma tissues. Therefore, they might be associated with metastasis. Conclusion:Further analysis of these differentially expressed metastasis associated genes will be helpful for understanding the molecular mechanism of malignant carcinoma.