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乙型肝炎病毒(ayw型)转基因小鼠的建立
引用本文:雷章恒,余宏宇.乙型肝炎病毒(ayw型)转基因小鼠的建立[J].第二军医大学学报,1997,18(3):201-204.
作者姓名:雷章恒  余宏宇
作者单位:[1]第二军医大学基础医学部生物学教研室和医学分子遗传学 [2]第二军医大学基础医学部病理?
基金项目:上海市青年科技启明星计划
摘    要:目的:建立遗传上稳定的、带有乙型肝炎病毒(HBV)ayw亚型的转基因小鼠品系。方法:通过对受精卵显微注射进行基因转移的途径制备转基因小鼠,以PCR-Southern杂产、ELISA、组织切片免疫组化和透射电镜等方法,分析所导入DNA在转基因小鼠体内的功能情况。结果:得到21只建立者小鼠,在它们的血清中检测到了HBsAG和HBeAg的存在,还在肝细胞中观察到了HBV样病毒颗粒。结论:HBV基因组DN

关 键 词:乙型肝炎病毒  转基因小鼠  动物模型

Generation of transgenic mice harbouring hepatitis B (ayw subtype)
Lei Zhangheng,Li Jianxiu,Yu Hongyu,Wang Xinmin,Sun Wei,Pan Xinghua,Hao Guangrong,Wang Xiaopeng,Fu Jiliang,Hu Yiping.Generation of transgenic mice harbouring hepatitis B (ayw subtype)[J].Academic Journal of Second Military Medical University,1997,18(3):201-204.
Authors:Lei Zhangheng  Li Jianxiu  Yu Hongyu  Wang Xinmin  Sun Wei  Pan Xinghua  Hao Guangrong  Wang Xiaopeng  Fu Jiliang  Hu Yiping
Abstract:Objective: To establish the transgenic mouse line harbouring intact hepatitis virus B (HBV) genome. Methods:The transgenic mice were generated by microinjection of the complete genome of HBV (ayw subtype) into fertilized eggs. The functional analysis of the HBV DNA integrated in the transgenic mice was carried out by using PCR Southern blot, ELISA, immunohistochemistry and transmission electronic microscopy. Results: Twenty one founder mice were obtained. HBsAg and HBeAg were detected in the serum from the founders and their offspring, and HBV like particles were found in hepatocytes. Conclusion: The transgenic mice with HBV (ayw subtype) have been generated. The HBV genes in the transgenic mice can be expressed and the HBV genome can been replicated and packaged.
Keywords:hepatitis B virus  transgenic mice  
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