异种肾移植间接体内模型的建立及病理学观察

目的：建立成熟稳定的异种肾移植（猪／人）间接体内模型，用以研究异种移植超急排斥反应的发生机制及对异种移植物所造成的损害。方法：猪肾２２只；为实验组；人肾８只，为对照组。利用体外灌注系统将人新鲜血经肝素抗凝、氧饱和后，３７℃条件下灌注离体肾脏。检测灌注前后血氧饱和度的变化和组织病理学改变。结果：两组肾脏灌注前、后的血氧饱和度变化无显著性差异。组织病理学检查发现：实验组的２２只猪肾在灌注开始后的５￣７

Establishment of an ex vivo model of pig to man renal xenotransplantation and pathological study

Objective:To establish an ex vivo model of pig to man renal xenotransplantation for further investigation of the mechanisms of hyperacute xenogeneic rejection(HXR) in discordant xenograft and for better understanding of the damage to the xenogeneic grafts .Methods:22 pig kidneys and 8 human kidneys (as control) were perfused in a perfusion system with oxygenerated heparinized human fresh blood.Changes of blood oxygen saturation levels were calculated before and after perfusion.Histological examinations were taken by light microscopy.Results:The same changes of oxygen saturation levels were observed between the experimental and control groups.The microscopic aspect of all the xenoperfused pig kidneys indicated an HXR within 5 7 min.In contrast ,all the 8 hemoperfused human kidneys of control group showed no signs of HXR.Conclusion:We have established an ex vivo model of renal xenotransplantation which is easy to manipulate and is much relevant to realistic clinical renal xenotransplantation.This ex vivo model with its high stability can be used for further study of pig to man renal xenotransplantation.