新城疫病毒HN基因真核表达质粒的构建及其抗肿瘤作用的初步研究

构建新城疫病毒血凝素神经氨酸酶基因真核表达质粒，并初步探索其在抗肿瘤治疗中的机制及应用价值。方法：用ＲＴ－ＰＣＲ从ＮＤＶ中克隆ＨＮ ｃＤＮＡ，并构建ＨＮ的真核表达载体，转染ＣＯＳ－７细胞表达，注射至小鼠Ｂ１６黑素瘤模型，检测其抗肿瘤作用。结果成功地克隆了ＮＤＶ ＨＮ ｃＤＮＡ，所构建的ｐｃＤＮＡ３－ＨＮ有良好的表达，动物实验显示，ｐｃＤＮＡ３－ＨＮ有增强荷瘤小鼠ＣＴＬ，ＮＫ杀伤活性的作用。结论ＮＤ

Construction of Newcastle disease virus hemagglutinin-neuraminidase (HN) gene and its antitumor immune response effects

Objective: To construct an eukaryotic expression plasmid containing gene coding for the hemagglutinin neuraminidase(HN) of Newcastle disease virus (NDV), and to study its mechanism and value in antitumor therapy. Methods: The HN cDNA was abtained from NDV with RT PCR and an eukaryotic expression vector of HN gene ( pcDNA3 HN ) was constructed. The antitumor effect was evaluated after injecting pcDNA3 HN into mice bearing B16 melanoma. Results: The HN cDNA of NDV was successfully cloned and pcDNA3 HN had a good expression in COS 7 cells. Animal experiments suggested that the pcDNA3 HN could significantly increase CTL and NK activity of tumor bearing mice. Conclusion:The eukaryotic expression plasmid containing the gene coding for the (HN) has the function of increasing CTL and NK activity of tumor bearing mice.