乙型肝炎病毒核心抗原转基因小鼠的建立

目的：建立乙型肝炎病毒核心抗原（ayw亚型）转基因小鼠模型。方法：采用受精卵显微注射的方法制备转基因小鼠，以PCR、免疫组化和H－E染色法分析导入基因在小鼠基因组中的整合，肝脏中的表达及肝组织的病理变化。结果：得到6只基因组中整合了核心抗原基因的首建者（founder）小鼠，其中1只在肝细胞核和胞浆均有HBcAg的表达。将肝脏中表达的小鼠继续传代，F1代10只小鼠中的4只小鼠肝细胞有HBcAg的表达。结论：建立了乙型肝炎病毒核心抗原转基因小鼠，核心抗原基因能够在小鼠肝脏中表达，并且可以遗传。

Development of transgenic mice harbouring hepatitis B virus core gene

Objective: To establish the transgenic mice model harbouring hepatitis B virus (HBV) core gene (ayw type). Methods:The transgenic mice were generated by microinjecting core gene into fertilized eggs.The analysis of the HBV core gene integration in the transgenic mice was carried out by PCR, immunohistochemistry and H E staining. Results:Six founder transgenic mice were obtained. HBcAg was detected in hepatocellular nuclei and cytoplasm in one founder transgenic mouse.Hepatitis B virus core gene integrated in all 10 F 1 offsprings. HBcAg was detected in 4 of 10. Conclusion:The HBV core gene is integrated in the mice genome and can be expressed.