| 包裹多烯紫杉醇PLGA-PEG纳米粒的制备及体外释放度考察 |
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| 引用本文： | 陈婷,鲁莹,林亚玲,黄景彬,涂晔,孙治国,刘俊杰,钟延强.包裹多烯紫杉醇PLGA-PEG纳米粒的制备及体外释放度考察[J].第二军医大学学报,2011,32(3):295-299. |
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| 作者姓名： | 陈婷 鲁莹 林亚玲 黄景彬 涂晔 孙治国 刘俊杰 钟延强 |
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| 作者单位： | 第二军医大学药学系药剂学教研室,第二军医大学药学系药剂学教研室 |
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| 基金项目： | 国家自然科学基金青年科学基金项目 |
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| 摘 要： | 目的制备包裹多烯紫杉醇PLGA-PEG纳米粒(DTX-NPs)并评价其体外释放行为。方法合成高分子聚合物PLGA-PEG-COOH并表征;以其作为载体,复乳溶剂挥发法制备隐形纳米粒;动态光散射粒径仪和透射电镜测定DTX-NPs的粒径分布、zeta电位及表面形态;采用HPLC法测定DTX-NPs的包封率及载药量;以pH 7.4磷酸缓冲盐溶液(PBS)作为释放介质,考察DTX-NPs的体外释放行为。结果 DTX-NPs平均粒径为(138.8±1.01)nm,zeta电位为(-13.74±3.54)mV,包封率(99.41%±0.29%),载药量(2.47±0.02)μg/mg,24 h突释量为49%。结论 DTX-NPs制备工艺简便可控,结果稳定,且其体外释放行为具有缓释性,能够在血液中长时间滞留,具有良好的应用前景,值得进一步研究。
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| 关 键 词： | 多烯紫杉醇 纳米粒子 聚乳酸羟基乙酸共聚物 体外释放 |
| 收稿时间： | 2010/12/17 0:00:00 |
| 修稿时间： | 3/3/2011 3:43:12 PM |
Preparation and in vitro release of docetaxel-loaded PLGA-PEG nanoparticles |
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| CHEN Ting,LU Ying,LIN Ya-ling,HUANG Jing-bin,TU Ye,SUN Zhi-guo,LIU Jun-jie,ZHONG Yan-qiang.Preparation and in vitro release of docetaxel-loaded PLGA-PEG nanoparticles[J].Academic Journal of Second Military Medical University,2011,32(3):295-299. |
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| Authors: | CHEN Ting LU Ying LIN Ya-ling HUANG Jing-bin TU Ye SUN Zhi-guo LIU Jun-jie ZHONG Yan-qiang |
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| Institution: | Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433, China |
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| Abstract: | Objective To prepare the docetaxel(DTX)-loaded nanoparticles based on poly(D,L-lactide-co-gly-colide)-block-poly(ethylene glycol)(PLGA-b-PEG-COOH)(DTX-NPs)and to evaluate their in vitro release property.Methods PLGA-PEG was successfully synthesized and double emulsion solvent evaporation technique was used to prepare DTX-NPs.The size distribution,zeta potential,and surface morphology of the prepared DTX-NPs were characterized by dynamic light scattering(DLS) and transmission electron microscopy(TEM);the ent... |
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| Keywords: | docetaxel nanoparticles poly(D L-lactide-co-glycolide) in vitro release |
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