膜雌激素对乳腺癌MCF-7细胞生长的调控及其对其表皮生长因子受体表达水平的影响

目的：探讨膜雌激素持续作用对乳腺癌细胞生长调控的影响及其可能的分子机制。方法采用MTT法测不同浓度的膜雌激素对乳腺癌细胞MCF-7生长的影响。分别采用MTT法和RT-PCR法分析不同的信号抑制剂调控后，膜雌激素对乳腺癌细胞MCF-7生长及其EGFR mRNA表达水平的影响。结果终浓度为10-7M和10-12M的膜雌激素持续作用能诱导乳腺癌MCF-7细胞的增殖。膜G蛋白藕联受体阻断剂苏拉明和EGFR阻断剂AG-1478能明显抑制膜雌激素对MCF-7细胞生长的诱导作用。苏拉明和AG-1478能抑制膜雌激素持续作用上调MCF-7细胞中EGFRmRNA表达的作用。结论雌激素膜持续效应能刺激乳腺癌细胞的增殖，这一作用是通过膜G蛋白藕联受体和EGFR通路来完成的。

The effects of EGFR expression and proliferation of breast cancer MCF-7 cells regulated by membrane estrogen

Objective To investigate the proliferation and the possible molecular mechanisms of the breast cancer cell regulated by membrane estrogen continuously. Method The MTT method was used to measure the the growth effects of breast cancer MCF-7 cell regulated by the different concentration of membrane estrogen. The MTT method and RT-PCR method were used respectively to analyze the breast cancer MCF-7 cell growth and the EGFR mRNA expression level regulated by membrane estrogen. Results The sustained action of membrane estrogen on the final concentration from 10-7M to 10-12M can induce breast cancer MCF-7 cell growth. The suramin（G protein-coupled receptor inhibitor） and AG-1478（EGFR inhibitor） can down-regulate the effect of MCF-7 cell growth stimulated by membrane estrogen. Suramin and AG-1478 can down-regulate the EGFR mRNA expression stimulated by membrane estrogen in the MCF-7 cell. Conclusion The continuous estrogen membrane effect can stimulate the proliferation of breast cancer cells, it is through the membrane G protein coupling receptor and EGFR pathway.