靶向GST-π、po1β基因的siRNA重组慢病毒对人食管鳞癌顺铂耐药逆转的体内实验研究

目的探讨靶向GST-π、polβ的siRNA重组慢病毒在裸鼠体内对人食管鳞癌顺铂耐药株耐药的逆转作用。方法采用皮下注射细胞法建立裸鼠人食管癌细胞细胞EC9706及其耐药细胞EC9706/cDDP移植瘤模型，观察其成瘤性和体内耐药性。利用靶向GST-π、polβ的siRNA重组慢病毒感染具有 MDR表型的裸鼠移植瘤，以期达到封闭GST-π、polβ的转录和表达，检测移植瘤细胞对药物的敏感性变化。结果 cDDP 联合靶向GST-π的siRNA慢病毒GSTsi2处理组中观察到移植瘤体积与cDDP组及PBS对照组比较，体积显著减小，表明经cDDP联合GSTsi2处理后，可明显恢复移植瘤细胞对 cDDP的敏感性。而在cDDP联合靶向polβ的siRNA慢病毒polsi1处理组中移植瘤体积与cDDP及PBS对照组比较无显著差异，移植瘤细胞对cDDP的敏感性无明显改变。结论靶向GST-π的重组慢病毒可有效逆转食管癌细胞的耐药性，靶向polβ的重组慢病毒逆转肿瘤细胞耐药的效果不明显。

In vivo study on the reversal of cisplatin resistance of esophageal cancer by Lentiviral vector of siRNA targeting to GST-π, po1β

Objective To investigate the reversal of cisplatin resistance of esophageal cancer by Lentiviral vector of siRNA targeting to GST-π, polβ in vivo. Methods EC9706 and EC9706/cDDP cells were subcutaneously injected into athymic nude mice to set up the animal models of grafted tumor. Injecting cDDP plus GSTsi2（polsi1） to xenografts derived from drug resistant cells to detect xenografts cells changes in drug sensitivity. Results The tumor volume of the group of cDDP plus GSTsi2 was lower than that of groups of PBS and cDDP; while the tumor volume of the group of cDDP plus polsi1 had no significant difference with groups of PBS and cDDP. Conclusion Injecting cDDP plus GSTsi2 to xenografts derived from drug resistant cells could inhibit its growth; while injecting cDDP plus polsi1 to xenografts could not inhibit its growth.