p16、p21、p27基因多态性与上皮性卵巢癌易感性关联的研究

目的探讨抑癌基因p16、p21和p27的多态性与上皮性卵巢癌易感性的关联.方法选取2007年3月-2011年4月间120例卵巢癌患者作为考察对象,同时选取120例健康女性人群作为对照组.分别采集外周静脉血5ml,提取基因组DNA,分析每个样本的基因型,并做统计学分析.结果两组的p16基因C540G的C/C、C/G和G/G基因型频率分布无显著性差异(P>0.05);两组的p16基因C580T的C/C、C/G和G/G基因型频率分布无显著性差异(P>0.05);两组p21基因的T/T、T/C和C/C基因型频率分布无显著性差异(P>0.05);两组的p27基因的V/V、V/G和G/G基因型频率分布有显著性差异(P<0.05).结论 p16基因和p21基因型的多态性上皮性卵巢癌的易感性有关;p27基因的V/V纯合型可能会增加卵巢癌的发病风险,值得临床关注.

The association of p16, p21, p27 polymorphism with susceptibility to ovarian cancer

Objective To study the association of p16, p21, p27 polymorphism with susceptibility to ovarian cancer. Methods 120 patients with epithelial ovarian cancer in our hospital during March 2007 to April 2011 were selected to study, and 120 healthy women as a control group. Results There were no significant difference（P〉0.05） between the frequency of C/C, C/G and G/G genotype in p16 C540G gene of ovarian cancer patients and the control group; there were no significant difference（P〉0.05） between the frequency of C/C, C/G and G/G genotype in p16 CfiSOT gene of ovarian cancer patients and the control group; there were no significant difference（P〉0.05） between the frequency of C/C, C/G and G/G genotype in p21 gene of ovarian cancer patients and the control group; there wase a significant difference（P〈0.05） between the frequency of V/V, V/G and G/G genotype in p27 gene of ovarian cancer patients and the control group. Conclusion Some association of p16 and p21polymorphism with susceptibility to ovarian cancer, and we found the V/V homozygous of p27 gene may increase the risk of ovarian cancer. It should be concerned in clinical diagnosis.