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| 环境激发吗啡条件性位置偏爱重现大鼠海马PSD-95的蛋白和DNA表达 | |
| 引用本文： | 朱华,韩军鸽,李兴彪,吴淑珍,陈鹏,罗良鸣.环境激发吗啡条件性位置偏爱重现大鼠海马PSD-95的蛋白和DNA表达[J].温州医学院学报,2008,38(3):233-236. |
| 作者姓名： | 朱华 韩军鸽 李兴彪 吴淑珍 陈鹏 罗良鸣 |
| 作者单位： | 1. 温州医学院法医学系,浙江,温州,325035 2. 温州市公安局瓯海分局,浙江,温州,325000 3. 温州市公安局,浙江,温州,325000 |
| 基金项目： | 浙江省教育厅资助项目，浙江省温州市科技局资助项目 |
| 摘 要： | 目的：检测吗啡条件性位置偏爱（conditioned place preference，CPP）重现大鼠海马区突触后致密质-95（PSD-95）的蛋白和DNA表达，观察PSD-95对吗啡成瘾记忆的影响。方法：建立大鼠吗啡CPP模型，自然消退后，通过环境来诱发CPP的重现，应用免疫组化和RT-PCR的方法，观察吗啡CPP重现组大鼠海马区PSD-95的表达，并与吗啡CPP消退组，生理盐水对照组进行比较。结果：吗啡CPP环境激发组，吗啡CPP消退组与生理盐水对照组相比，以及吗啡CPP环境激发组与吗啡CPP消退组相比，海马区PSD-95的表达明显降低，差异具有高度显著性（P〈0．01）。结论：吗啡CPP重现和消退大鼠海马区PSD-95表达明显降低，海马区PSD-95可能未参与成瘾记忆。 |
| 关 键 词： | 吗啡 条件性位置偏爱 环境激发 海马 突触后致密质-95 |
| 文章编号： | 1000-2138(2008)03-0233-04 |
| 修稿时间： | 2007年10月18 |
Expression of PSD-95 protein and DNA in Hippocampal of rat with morphine conditioned place preference after environment induced reinstatement |
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| ZHU Hua,HAN Jun-ge,LI Xing-biao,WU Shu-zhen,CHEN Peng,LUO Liang-ming.Expression of PSD-95 protein and DNA in Hippocampal of rat with morphine conditioned place preference after environment induced reinstatement[J].Journal of Wenzhou Medical College,2008,38(3):233-236. | |
| Authors: | ZHU Hua HAN Jun-ge LI Xing-biao WU Shu-zhen CHEN Peng LUO Liang-ming |
| Institution: | ZHU Hua , HAN dun-ge, LI Xing-biao, WU Shu-zhen, CHEN Peng,LUO Liang-ming. (Department of Forensic Medicine, Wenzhou Medical College, Wenzhou, 325035) |
| Abstract: | Objective:To observe the expression of protein and DNA of postsynaptic density protein-95(PSD-95) in hippocampus of rats with morphine conditioned place preference(CPP) after environment induced reinstatement,and to evaluate the influence of PSD-95 on addiction memory.Methods:CPP rat model was established with morphine injection and reinstatement was induced by environment after extinction of CPP.The expression of PSD-95 protein and DNA in hippocampus of rats in reinstatement group of morphine CPP were observed,and was compared with those in extinction group of morphine CPP and control group.Results:The expression of PSD-95 in hippocampus was significantly decreased in rats in reinstatement and extinction group of morphine CPP compared with those in control group(P<0.01),and it was signifi-cantly decreased in reinstatement group compared with those in extinction group(P<0.01).Conclusion:The expression of PSD-95 in hippocampus is significantly decreased in rats in reinstatement and extinction group of morphine CPP.PSD-95 in hippocampus has take part or not in addict memory. |
| Keywords: | morphine conditioned place preference environmental induce hippocampus postsynaptic density protein-95 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |