IFN-γ基因多态性与HBV感染不同结局之间的关系

目的：探讨IFN-γ基因多态性与HBV感染不同临床转归之间的相关性。方法：应用聚合酶链反应-序列特异性引物法（PCR-SSP）检测60例自限性HBV感染者（SR组）和118例慢性持续性HBV感染者包括无症状HBV携带者（AsC组）36例和进展性肝病者82例（慢性乙型肝炎50例、乙肝后肝硬化32例）]的IFN-γ基因＋874 T→A、＋2109 A→G两个位点的多态性，比较各组间基因型和等位基因频率。结果：①＋874 T→A位点多态性中，慢性持续性感染者总从基因型频率比SR组显著升高（P〈0．05），差异有显著性。等位基因A的频率，进展性肝病组均显著高于SR组（P〈0．05）。②＋2109 A→G位点多态性中，进展性肝病组总GG基因型显著高于SR组（P〈0．05），而肝硬化和慢乙肝组之间，SR和AsC组之间差别无显著性。结论：IFN-γ基因＋874 T→A、＋2109 A→G两个位点的多态性，与HBV感染不同结局之间有关，携带＋874 T→A从基因型和A等位基因的HBV感染者容易发展为慢性，A等位基因更趋向于进展性肝病，而携带＋2109 A→GGG基因型患者则容易进展为慢性乙型肝炎甚至肝硬化。

Relationship between IFN- y gene polymorphism and clinical outcomes of hepatitis B virus infection

Objective： To explore the relativity between IFN- γ gene polymorphism and the clinical outcomes of HBV infection, Methods：A case-control study and PCR-SSP was used to detect IFN- γ ＋874 T → A and ＋2109 A → G polymorphism of 118 chronic HBV infected patients and 60 subjects which spontaneously recovered from HBV infection（SR group）. The chronic HBV infection group included 36 asymptomatic HBV carriers （AsC group）, 50 chronic hepatitis B and 32 HBVrelated liver cirrhosis. Results： The AA genotypo frequency of IFN- γ ＋874 T → A was significantly higher in chronic HBV infection group than that of SR group（P〈0.05）. The frequency of A allele was significantly higher in all advanced liver disease group （including chronic hepatitis B and liver cirrhosis） than that of SR group （P〈0.05）. The 03 genotype frequency of IFN- γ ＋2109 A → G was significantly higher in all advanced liver disease group than that of SR group （P〈0.05）. Conclusion： The results suggest that IFN- γ ＋874 T → A AA genotype and A allele may be associated with susceptibility of chronic HBV infection. The A allele may be related with advanced liver diseases. IFN-γ ＋2109 A → G 03 genotype may be related with susceptibility of chronic hepatitis B, even liver cirrhosis.