| 单精子卵胞浆内注射术中不同来源精子的处理方法 |
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| 引用本文： | 黄学锋,郑菊芬,李澄棣,叶碧绿,林金菊,周颖,赵军招,周红卫.单精子卵胞浆内注射术中不同来源精子的处理方法[J].温州医学院学报,2001,31(4):205-207. |
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| 作者姓名： | 黄学锋 郑菊芬 李澄棣 叶碧绿 林金菊 周颖 赵军招 周红卫 |
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| 作者单位： | 温州医学院第一附属医院生殖医学中心, |
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| 摘 要： | 目的：介绍单精子卵胞浆内注射术（ICSI）中不同来源精子的洗涤处理方法。方法：轻度少、弱精精液或正常精液精子，采用Percoll梯度离心法或直接离心加上游法处理；严重少、弱精液精子或无精子症的附睾液精子，采用直接离心法处理；无精子症的睾丸组织内精子，采用组织磨碎加直接离心法处理。结果：三种方法处理后，均获得足够的活动精子用于ICSI治疗。ICSI操作均能顺利进行。采用直接离心法获得的精子行ICSI可以获得相似的治疗结果。结论：不同来源精子都可以经直接离心法处理，并能有效用于ICSI治疗中。
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| 关 键 词： | 单精子卵胞浆内注射术 精子 洗涤 ICSI 辅助生育技术 |
| 文章编号： | 1000-2138(2001)04-0205-03 |
| 修稿时间： | 2000年8月21日 |
Sperm preparation methods of ejaculated, epididymal and testicular spermatozoa in
intracytoplasmic sperm injection |
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| HUANG Xue feng,ZHENG Ju fen,LI Cheng di,et al..Sperm preparation methods of ejaculated, epididymal and testicular spermatozoa in
intracytoplasmic sperm injection[J].Journal of Wenzhou Medical College,2001,31(4):205-207. |
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| Authors: | HUANG Xue feng ZHENG Ju fen LI Cheng di |
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| Institution: | HUANG Xue feng,ZHENG Ju fen,LI Cheng di,et al. Reproductive Medicine Unit,the First Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000 |
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| Abstract: | Objective:To describe sperm preparation methods of ejaculated, epididymal and testicular spermatozoa in intracytoplasmic sperm injection (ICSI).Methods:The sperm preparation methods included Percoll or swim up for spermatozoa from mild oligoasthenospermia or normal semen, direct centrifugation for those from severe oligoasthenospermia or epididymal aspirates, and direct centrifugation after mincing of testicular tissue for those from testicular biopsies.Results:Enough mobile spermatozoa could be activated from all three methods and used in ICSI procedure. The direct centrifugation method achieved comparable ICSI results.Conclusion:The direct centrifugation method could be used for preparation of spermatozoa from different sources in ICSI procedure. |
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| Keywords: | intracytoplasmic sperm injection (ICSI) sperm washing |
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