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| 免疫分子佐剂ctxB在pVAX1中的初步应用 | |
| 引用本文： | 梅丽琴,曲云鹏,麻健丰,江明华.免疫分子佐剂ctxB在pVAX1中的初步应用[J].温州医学院学报,2012,42(1):24-26. |
| 作者姓名： | 梅丽琴 曲云鹏 麻健丰 江明华 |
| 作者单位： | 1. 温州医学院附属口腔医院,儿童口腔科,浙江,温州,325027 2. 中国医科大学辽阳中心医院,口腔科,辽宁,辽阳 3. 温州医学院附属口腔医院,口腔修复科,浙江,温州,325027 4. 温州医学院附属第二医院,检验科,浙江,温州,325027 |
| 摘 要： | 目的 构建霍乱毒素B亚单位编码基因(ctxB)的真核表达质粒pVAX1-ctxB,并观察其在真核细胞中的蛋白表达情况.方法 利用基因重组技术构建真核表达质粒pVAX1 -ctxB,通过脂质将其转染至CHO细胞中诱导蛋白表达,GM1 -ELISA法检测其蛋白表达产物.结果 重组质粒pVAX1-ctxB经酶切可得到与ctxB大小相同的片段,GM1 -ELISA法证实经转染的CHO细胞可分泌霍乱毒素B亚单位(ctxB)蛋白.结论 成功构建重组表达质粒pVAX1- ctxB,其蛋白表达产物具有ctxB蛋白活性,为下一步加强基因防龋疫苗效价奠定了基础. |
| 关 键 词： | 分子佐剂 霍乱毒素B亚单位 pVAX1 霍乱毒素 基因 蛋白表达 |
Primary application of molecular-adjuvant ctxB in pVAX1 |
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| MEI Liqin,QU Yunpeng,Ma Jianfeng,JIANG Minghua.Primary application of molecular-adjuvant ctxB in pVAX1[J].Journal of Wenzhou Medical College,2012,42(1):24-26. | |
| Authors: | MEI Liqin QU Yunpeng Ma Jianfeng JIANG Minghua |
| Institution: | .Department of Pediatric Dentistry,the Affiliated Stomatology Hospital of Wenzhou Medical College,Wenzhou,325027 |
| Abstract: | Objective:To evaluate the expression of recombinant plasmid pVAX1-ctxB in mammalian cell.Methods: The eukaryotic plasmid carrying encoding gene of ctxB was con-structed and introduced into CHO cells by lipofectamine reagent.The transient protein expression was detected with immunochemistry technique in CHO cells.Results: The positive expression of ctxB was detected in plasma of the cells which were transfected with recombinant plasmid pVAX1-ctxB.The cells which were transfected with pVAX1 were negative for ctxB expression.Conclusion: CtxB can be translated and expressed in CHO cells after transfected with recombinant plasmid pVAX1-ctxB.The expressed protein is located in the plasma to combine with the ctxB antibody. |
| Keywords: | molecular-adjuvant ctxB pVAX1 cholera toxin gene protein expression |
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