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低氧对小鼠旷场行为的影响
引用本文:尉晓娜,谢于鹏,夏誉,何金彩.低氧对小鼠旷场行为的影响[J].温州医学院学报,2012,42(1):17-19.
作者姓名:尉晓娜  谢于鹏  夏誉  何金彩
作者单位:温州医学院附属第一医院,睡眠中心,浙江,温州,325000
基金项目:温州市科技局国际合作项目
摘    要:目的 观察低氧对小鼠行为的影响.方法 6月龄C57BL/6J小鼠按每日低氧时间不同随机分为低氧1组(8h/day,n=9)、低氧2组(12 h/day,n=8)、低氧3组(24h/day,n=9)及正常氧浓度对照组(n=8).低氧组小鼠在低氧舱中暴露8周后,检测所有小鼠在旷场实验中的行为变化.结果 与对照组比较,低氧后小鼠在旷场实验的中央格、总格、外周格穿越次数及相应路程均明显增加,差异具有统计学意义(P<0.05),提示低氧可使小鼠水平活动度及兴奋性异常增加;与对照组比较,低氧组小鼠体质量均下降,心重指数均增加,差异具有统计学意义(P<0.05),低氧3组小鼠体质量下降最明显.结论 低氧可引起小鼠活动度增加及体质量下降.

关 键 词:小鼠  低氧暴露  旷场实验

Effect of hypoxic exposure on behavior in Open Field Test in mice
WEI Xiaona,XIE Yupeng,XIA yu,HE jincai.Effect of hypoxic exposure on behavior in Open Field Test in mice[J].Journal of Wenzhou Medical College,2012,42(1):17-19.
Authors:WEI Xiaona  XIE Yupeng  XIA yu  HE jincai
Institution:.Department of Sleep Center,the First Affiliated Hospital of Wenzhou Medical College,Wenzhou,325000
Abstract:Objective:To assess the effect of hypoxic exposure on Open Field Test behavior in mice.Methods: 6-month-old C57BL/6J male mice were divided into four groups:the control mice(n=8) were housed in room air in normal situations without any treatment;hypoxia 1(8 h/day,n=9),hypoxia 2(12 h/day,n=8),hypoxia 3(24 h/day,n=9) mice were housed in designed hypoxia chamber for 8 weeks.After the hypoxic exposure,all mice were tested for Open Field Test behavior and the weight and heart index were also measured.Results: In Open Field Test,compared with the control group,hypoxia increased number of crossing and distance in peripheral area,in central area and in the total area of open field.It indicated mice were hyperactive following hypoxic exposure.Compared with the control group,hypoxic exposure decreased the weight of mice(P <0.05) and increased heart index(P <0.05).Conclusion: Hypoxia can lead to increases in horizontal activity and decrease in weight in mice.
Keywords:mice  hypoxic exposure  Open Field Test
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