头颈部鳞状细胞癌DNA含量与临床生物学行为的关系

目的：研究头颈部鳞状细胞癌（HNSCC）DNA倍体的变化与临床生物学行为的关系。方法：选择40例未经治疗的HNSCC患术后新鲜肿瘤标本，采用流式细胞仪（FCM）测定DNA含量的3个主要参数，异倍体（aneuploid),DNA指数（DNA Index,DI)和S期细胞比例（S－phase fraction,SPF)，探索3个参数与肿瘤组织学分级，临床分期以及淋巴结转移之间的关系。结果：40例未经治疗的HNSCC中，异倍体检出率为62．5％（25／40）。DI与淋巴结转移无关，与肿瘤组织学分级和肿瘤临床分期有关（P＜0．01）。SPF与肿瘤组织分级无关，与临床分期及淋巴结转移有关（P＜0．01）。结论：DNA异倍体是HNSCC的重要特征之一；DI和SPF可作为预测头颈部鳞状细胞癌生物学特性的重要指标。

The relations between DNA contents and biological behavior in head and neck squamous cell carcinoma

Objective:To study the relation between DNA contents and biological behavior in head and neck squamous cell carcinoma(HNSCC).Methods:Fresh specimens from 40 previously untreated patients with head and neck squamous cell carcinoma (HNSCC)were selected. Aneuploid, DNA index(DI)and S phase fraction(SPF)were measured using flow cytometry(FCM).The association of these parameters with histologic grades,clinical stage and lymph node metastasis were studied.Results:It showed that the ratio of aneuploid was 62.25%(25/40).DI was not correlated with lymph node metastases, but correlated with histologic grades and clinical stages (P<0.01).SPF was correlated both with clinical stages and lymph node metastasis(P<0.01).SPF had no relation with histologic grades.Conclusion:DNA aneuploid is one of the most important specialities in HNSCC,meanwhile DI and SPF can be regarded as significant predictors of biologic characteristics in HNSCC.