微针阵列经皮促透NGF对家兔皮肤感觉神经再生的影响

目的探索NGF（Nerve growth factor,NGF）促进移植皮肤感觉恢复的微创给药方法。方法亚甲蓝染色经微针阵列处理后的离体兔皮,观察微孔的分布和排列,冰冻切片HE染色观察微孔的深度。兔皮片移植模型分别采用直接涂布、微针促透和皮内注射给药,ELISA法检测皮肤匀浆液中NGF的含量。皮肤组织切片免疫组化染色观察NGF的分布和神经纤维的再生情况。结果微针阵列处理后离体兔皮微孔排列均匀,深度可达真皮浅层。动物模型给药6h后,微针促透组和皮内注射组皮肤组织匀浆液中NGF含量分别高于直接涂布组5.48倍和6.45倍,差异均有统计学意义（P〈0.05）;NGF免疫组化染色显示,微针促透组真皮组织中有大量NGF存在,且分布均匀;动物模型给药30d后,皮肤组织S100免疫组化染色显示,微针促透组和皮内注射组真皮中可见再生的神经纤维,两组差异无统计学意义（q=2.36,P〉0.05）,并均显著高于直接涂布组（q1=7.74,q2=6.15,P〈0.05）。结论通过微针阵列可使NGF透过兔皮肤角质层到达真皮,促进兔移植皮肤感觉重建。

Effect of Microneedle Arrarys Transdermal Delivering Nerve Growth Factor on Promoting the Rehabilitation of Transplanted Skin Sensation in Rabbits

Objective To explore a minimally invasive administration method of NGF to promote rehabilitation of the transplanted skin sensation.Methods Following treatment of microneedle arrays in skin of rabbit,the distribution and the depth of microporous were visualized using methylene blue staining of the skin and HE staining of cryostat sections;Three different methods: Microneedle mediated delivery,intradermal injection and direct coating were applied in this research,NGF concentration of the skin homogenate of r...