桃红四物汤治疗原发性痛经实验研究

目的：观察桃红四物汤治疗痛经的作用，探讨其机制。方法：①观察桃红四物汤对热板法所致昆明种雌性小鼠疼痛的影响。②复制缩宫素致Wistar雌性大鼠痛经模型，观察桃红四物汤不同剂量对大鼠扭体次数、扭体发生率、血浆β-内啡肽（β-endorphin，β—EP）及子宫组织前列腺素F2a（prostaglandin F2a，PGF2a）的影响。结果：与模型组比较，桃红四物汤各剂量组小鼠痛阈均显著提高（P〈0．01），痛经大鼠30rain内扭体次数显著减少（P〈0．01），扭体发生率降低；大鼠子宫组织PGF2。含量显著降低（P〈0．01）；与模型组比较，桃红四物汤高、中剂量组大鼠血浆β—EP含量显著升高（P〈0．05），与田七痛经胶囊组及阿司匹林组比较，差异无显著性（P〉0．05）。结论：①桃红四物汤有明显的镇痛作用；②有调节大鼠血浆β—EP及子宫组织前列腺素分泌的作用。

An Experimental Study on Effects of Taohong Siwu Decoction for Primary Dysmenorrhea

Objective： To observe the effects of Taohong Siwu Decoction in treating primary dysmenorrhea, and explore its mechanism. Methods： （1）A hot plate test was employed to observe the therapeutic effects of Taohong Siwu Decoction on pain in female Kunming mice. （2）The effects of different dosage of Taohong Siwu Decoction on writhing frequency, writhing incidence, the plasma level of β-endorphin （13-EP） and the homogenate level of prostaglandin F2a（PGF2a） in the uterus were observed in oxytocin-induced female rats. Results： As ,compared with that of model group, the pain threshold was significantly increased in mice of groups treated with different dosage of Taohong Siwu Decoction, the writhing frequency and writhing incidence during 30 minutes were signifi- candy decreased in rats （P〈0.01）, the level of PGF2a in rat uterus was significantly decreased （P〈0. 01）. The plasma level of β-EP was significantly increased in rats of groups treated with high and middle dosage of Taohong Siwu Decoction compared with that of model group, but there was no significant difference in the rat plasma level of β-EP between the groups treated with high and middle dosage of Taohong Siwu Decoction and groups treated with Tianqi Tongjing Capsule and aspirin （P〉0.05）. Conclusion： The analgesic effect of Taohong Siwu Decoction is significant, and Taohong Siwu Decoction can regulate the secretion of plasma β-EP and uterus PGF2a.