蛛网膜下腔注射P物质在电针治疗大鼠局灶性脑缺血中的作用

目的：探索蛛网膜下腔注射P物质（substance P，SP）在电针治疗局灶性脑缺血过程中的作用。方法：将40只Wistar大鼠随机分为对照组、9．0g／L氯化钠注射液（normal saline，NS）组、SP组、电针组和SP＋电针组，每组各8只。采用大脑中动脉结扎（middle cerebral artery occlusion，MCAO）复制局灶性脑缺血模型，NS组和SP组大鼠分别在MCAO后24h蛛网膜下腔注射NS和SP，在MCAO手术同时进行电针治疗，SP＋电针组在电针治疗基础上于MCAO后24h蛛网膜下腔注射SP。以氯化三苯基四氮唑（triphenyl tetrazolium chloride，TTC）染色法测定脑缺血面积，免疫组织化学法检测脑组织SP含量。结果：与对照组比较，SP组和电针组在MCAO后24h神经行为学评分显著降低（P〈0．05）；SP十电针组神经行为学评分显著低于SP组（P〈0．01）。与对照组比较，电针组和SP＋电针组脑梗死面积显著缩小（P〈0．01）。SP＋电针组大鼠大脑皮质和海马中SP含量显著高于对照组（P〈0．05，或P〈0．01）。结论：蛛网膜下腔注射SP可增强电针治疗局灶性脑缺血的疗效。

The Role of Injecting Substance P into the Subarachnoid Space during the Treatment of Focal Cerebral Ischemia with Electroacupuncture in Rats

Objective： To research the role of injecting substance P （SP） into the subarachnoid space during electroacupuncture （EA） in the treatment of focal cerebral ischemia in rats. Methods： Forty Wistar rats were averagely randomized into control group, normal saline （NS） group, SP group, EA group and SP＋EA group. The focal cerebral ischemia model was induced by the method of middle cerebral artery occlusion （MCAO）. The rats in NS group and SP group were injected NS and SP into the subarachnoid space, respectively. The rats in EA group were treated with EA therapy since the MCAO operation. The rats in SP＋EA group were treated with EA therapy and the injection of SP into the subarachnoid space 24 hours after MCAO. The ischemic area in the cerebrum was detected using triphenyl tetrazolium chloride （TTC） staining. The contents of SP in the cerebrum were detected using immunohistochemistry method. Results： Compared with those in control group, the neuroethology scores in SP group and EA group were significantly decreased 24 hours after MCAO, and the neuroethology scores in SP＋EA group were signifi- cantly lower than those in SP group （P〈0.01）. Compared with that in control group, the ischemic areas in EA group and SP＋EA group were significantly decreased （P〈0.01）. The contents of SP in the corfex and the hippocampus of SP＋EA group were significantly increased compared with those of control group （P〈0. 05 ,or P〈0.01）. Conclusion： The injection of SP into the subarachnoid space can improve the therapeutic effects of EA in treating focal cerebral ischemia.