反相高效液相色谱法测定木瓜药材及饮片中齐墩果酸和熊果酸含量

目的建立木瓜药材及饮片中齐墩果酸和熊果酸的含量测定方法。方法采用反相高效液相色谱方法：Shim-pack CLC-ODS（M）色谱柱（250 mm×4.6 mm,5μm）,柱温20℃;流动相为甲醇-水-冰乙酸-三乙胺（265∶35∶0.1∶0.05）,流速0.6 ml/min;检测波长210 nm。结果齐墩果酸在0.162－3.310μg范围内（r=0.999 8）,熊果酸在0.195－3.910μg范围内（r=0.999 4）与峰面积线性关系良好;齐墩果酸平均回收率为97.7%,RSD=2.0%（n=9）,熊果酸平均回收率为98.4%,RSD=2.5%（n=9）。木瓜药材及饮片齐墩果酸含量为0.248%－0.454%,熊果酸含量为0.103%－0.508%。结论所建立的齐墩果酸与熊果酸含量测定方法操作简便、准确、重现性好,可用于不同产地木瓜药材及饮片的质量控制。

Content Determination of Oleanolic Acid and Ursolic Acid in the Medicinal Materials and Prepared Slices of Fructus Chaenomelis by Reversed Phase-High Performance Liquid Chromatography

Objective To establish a method for the determination of oleanolic acid and ursolic acid in the medicinal materials and prepared slices of Fructus Chaenomelis.Methods A method of reserved phase-high performance liquid chromatography(RP-HPLC) was employed.Oleanolic acid and ursolic acid were separated on Shim-pack CLC-ODS(M)(250 mm×4.6 mm,5 μm).The mobile phase consisted of methanol-water-glacial acetic acid-triethylamine(265∶35∶0.1∶0.05),the column temperature was kept at 20℃,the flow rate was 0.6ml/min,and the detective wavelength was set at 210 nm.Results The linear relationship ranged from 0.162 to 3.310 μg(r=0.999 8) for oleanolic acid,and 0.195 to 3.910 μg(r=0.999 4) for ursolic acid.The average recovery rates were 97.7%(RSD=2.0%,n=9) for oleanolic acid,and 98.4%(RSD=2.5%,n=9) for ursolic acid,respectively.The contents of oleanolic acid and ursolic acid in Fructus Chaenomelis and its prepared slices ranged from 0.248% to 0.454%,and 0.103% to 0.508%,respectively.Conclusion The established method of content determination for the oleanolic acid and ursolic acid is simple,accurate,reproducible,and suitable for the quality control of the medicinal materials and prepared slices of Fructus Chaenomelis from different places.